This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.
Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
15
Participants consumed a diet that was high in fat and high in energy
Change in glucose concentration
Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L.
Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Change in insulin concentration
Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L.
Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers
Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min.
Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Change in skeletal muscle insulin signalling
AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units.
Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Change in markers of microvascular function
endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.
Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Change intramuscular triglyceride content
Intramuscular triglyceride content was determined by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.
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Time frame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)