The purpose of this study is to examine whether breaking up prolonged sitting with short regular bouts of walking can reduce blood sugar and cholesterol levels after eating, which are risk markers for Type 2 diabetes and heart disease. This study will compare these responses in normal-weight versus overweight/obese South Asian adults.
Participants will attend a preliminary testing session where height, weight, body fat and waist circumference will be measured. They will be familiarised with a motorised treadmill that will be used during the experimental visit below and will take part in an exercise test to determine a light-intensity walking speed for the experimental visit. Participants will then attend two experimental visits in a random order: 1. Prolonged sitting (SIT): Participants will remain seated for 5 hours and instructed to reduce excessive movement. 2. Breaking up sitting with walking breaks (INT-SIT): Participants will rise from the seated position every 30 minutes throughout the experimental period to walk on a motorised treadmill at a light intensity for 5 minutes. After performing walking activity, they will return to the seated position. In addition, blood sample will be taken at the resting position just before each physical activity bout. The activity breaks will be undertaken on 9 occasions, providing a total of 45 min of light-intensity activity. During each visit, blood pressure will be taken 11 times and blood samples will be taken 10 times. Participants will be given a breakfast and lunch meal to consume.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
54
See Breaking up sitting with walking breaks arm description
See Prolonged sitting arm description
University of Bedfordshire
Bedford, Bedfordshire, United Kingdom
Postprandial Glucose
Incremental area under the curve for each 5 h condition will be calculated for glucose
Time frame: Measured over the 5 hour condition period
Postprandial Triglycerides
Incremental area under the curve for each 5 h condition will be calculated for triglycerides
Time frame: Measured over the 5 hour condition period
Postprandial Insulin
Incremental area under the curve for each 5 h condition will be calculated for insulin
Time frame: Measured over the 5 hour condition period
Metabolic Load Index
Incremental area under the curve for each 5 h condition will be calculated for the Metabolic Load Index (MLI). The MLI will be calculated using the following equation: MLI (mmol.L-1) = blood glucose (mmol.L-1) + TAG (mmol.L-1). Lower values of MLI are considered better for cardiometabolic health!
Time frame: Measured over the 5 hour condition period
Resting Energy Expenditure
Incremental area under the curve for each 5 h condition will be calculated for resting energy expenditure
Time frame: Measured for the 5 hour condition period
Resting Fat Oxidation
Incremental area under the curve for each 5 h condition will be calculated for resting fat oxidation
Time frame: Measured for the 5 hour condition period
Resting Carbohydrate Oxidation
Incremental area under the curve for each 5 h condition will be calculated for resting carbohydrate oxidation
Time frame: Measured for the 5 hour condition period
Mean Arterial Pressure
Mean Arterial Pressure (MAP) over the 5 h conditions. The following formula will be used to calculate MAP, where P is blood pressure (systolic or diastolic): MAP = PDiastolic + 1/3 (PSystolic - PDiastolic).
Time frame: Measured over the 5 hour condition period
Heart Rate
Mean heart rate over the 5 h conditions
Time frame: Measured over the 5 hour condition period
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