Oxytocin Receptor Expression in Pregnancy

N/AActive Not RecruitingNCT03907735

Oregon Health and Science University30 enrolled

Overview

This study aims to determine when during gestation oxytocin receptor (OXTR) expression increases in the myometrium, which will contribute new insight to the management of obstetric hemorrhage and our understanding of normal and abnormal labor.

In this descriptive study, we will obtain myometrial tissue samples by core needle biopsy under ultrasound guidance in anesthetized women immediately following surgical abortion and from controls, all of whom are undergoing a separate planned obstetric/gynecologic surgery. Tissue studies will be performed on these biopsy samples to examine oxytocin receptor expression and function.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Post-Abortion Hemorrhage, Obstetric

Interventions

Myometrial tissue sample collectionOTHER

Myometrial tissue samples by core needle biopsy under ultrasound guidance

Eligibility

Sex: FEMALEMin age: 18 YearsMax age: 50 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Women age 18-50 * Gestational age 14-26 weeks; or 6-13 weeks, 37-42 weeks, or non-pregnant (controls) * Undergoing another planned surgical procedure with anesthesia (suction D\&C, D\&E, gravid hysterectomy, cesarean section, or laparoscopic tubal ligation) * Able to give informed consent in English or Spanish Exclusion Criteria: To optimize biopsy safety, we will exclude patients with anticipated difficult cases, that have additional risks of hemorrhage, or those with features that limit ultrasound visualization. Increased risk of hemorrhage: * Anemia (Hgb \< 9) * Anticoagulation, bleeding disorder, or coagulopathy * Multiple gestation * Infection or sepsis For ultrasound-guided procedures: * Obese, BMI ≥ 40 * History of ≥2 cesarean sections * Large fibroids or uterine anomalies obstructing view * Other poor visualization * Thin myometrium \< 1cm

Locations (1)

Oregon Health & Science University

Portland, Oregon, United States

Outcomes

Primary Outcomes

Oxytocin receptor expression

Measure of oxytocin receptor expression. Perform quantitative RT-PCR on mRNA extracted from biopsy samples obtained from subjects at the end of their procedure (within 30 minutes).

Time frame: 30 minutes

Secondary Outcomes

Function of myometrial oxytocin receptor

Assessment of the function of the myometrial oxytocin receptor. Measure intracellular calcium in cultured myometrial cells after activating the receptor with oxytocin from biopsy samples obtained from subjects at the end of their procedure (within 30 minutes).

Time frame: 30 minutes

Data from ClinicalTrials.gov

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