Quantification of Estradiol's Impact on Nucleotides in Cellular Populations of the Lower GI Tract

Inclusion Criteria: * Healthy cisgender pre-menopausal female participants between the ages of 18 and 49 years, inclusive on the date of screening (Healthy is defined as no irregular menstrual cycles or clinically relevant abnormalities identified by a detailed medical history, full physical examination, including blood pressure and pulse rate measurement, and clinical laboratory tests. * Regular menstrual cycles defined as at least 1 day of menses occurring every 21-35 days) * Estimated calculated creatinine clearance (eCcr) of at least 60 mL/min by the Cockcroft-Gault formula where: eCcr (female) in mL/min = \[(140 - age in years) x (weight in kg) x 0.85\] / (72x serum creatinine in mg/dL). * Negative serum pregnancy test at screening * All participants should be using at least one of the following methods of contraception\* from the screening visit through 72 hours prior to inpatient admission (at which time the women will be asked to remain abstinent until after their follow-up visit): 1. Non continuous systemic hormonal contraceptives that permit intermittent menstruation 2. IUD (non-hormonal intrauterine device) placed at least 1 month prior to study enrollment 3. Bilateral tubal ligation (Sterilization) 4. Vasectomized male partners 5. Condom + Spermicide 6. \*Unless engaged in sexual activity with female only sex partners or abstinent for at least 3 months prior with no intention of becoming sexually active during the study period. Any history of recent or present concomitant male sex partners will be addressed and ruled out in the context of screening participants for eligibility for the protocol * Evidence of a personally signed and dated informed consent document indicating that the subject has been informed of all pertinent aspects of the trial. * Willing and able to comply with scheduled visits, treatment plan, laboratory tests, and other trial procedures. * Subject must be willing to abstain from sexual intercourse, and all and intrarectal objects and products for at least 72 hours prior to Sampling #1 until study completion. * Subject must be HIV-1 and Hepatitis B and C negative as documented on screening labs. * Subject must not be actively involved in the conception process and must be non-lactating. * Subject must be able to swallow pills and have no allergies to any component of the study product Exclusion Criteria: * Evidence or history of clinically significant hematological, renal, endocrine, pulmonary, gastrointestinal, cardiovascular, hepatic, psychiatric, neurologic, or allergic disease (including documented drug allergies, but excluding untreated, asymptomatic, seasonal allergies at time of dosing). * Participants with a history of hysterectomy * Participants who are pregnant, possibly pregnant or lactating * History of febrile illness within five days prior to first dose. * Any condition possibly affecting drug absorption (eg, gastrectomy or other significant alterations of the gastrointestinal tract) * A positive urine drug screen. * An untreated-positive test for syphilis, gonorrhea, or Chlamydia at screening. * Any clinically relevant laboratory chemistry or hematology result Grade 2 or greater according to the Division of AIDS Laboratory Grading Tables * Treatment with an investigational drug within 4 months preceding the first dose of study product. * History of regular alcohol consumption exceeding 14 drinks (1 drink = 5 ounces (150 mL) of wine or 12 ounces (360 mL) of beer or 1.5 ounces (45 mL) of spirits) per week. * Participation in a clinical trial involving rectal biopsies within 6 months preceding the first dose of trial medication. * Blood donation of approximately 1 pint (500 mL) within 56 days prior to dosing. * Any condition which, in the opinion of the investigator, is likely to interfere with follow-up or ability to take the study medication appropriately. * Unwilling or unable to comply with the dietary and concomitant drug restrictions in regard to study drug administration as outlined in the study procedures and prohibited medications sections. * Women utilizing continuous hormonal contraception options such as Seasonique, injectables, implants, and hormonal IUDs