Serum Amyloid A in Women With Unexplained Infertility

Ain Shams Maternity Hospital90 enrolled

Overview

High follicular fluid amyloid A level is associated with reduced pregnancy rate. The aim of this study was to find an association between serum level of amyloid A and unexplained infertility.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Infertility

Interventions

Serum amyloid A levelOTHER

For determination of SAA level, a 5-ml sample of venous blood will be taken from each participant under aseptic conditions, and centrifuged at 2500 g for 15 minutes at 4°C c. separated into serum aliquots,and stored frozen at -80°C until analysis of SAA ,which will be done by a double-antibody sandwich Enzyme Linked Immunosorbent Assay (ELISA) kits supplied by cusabio,USA according to manufacturers instruction .Each sample was tested in duplicate and the average will be taken. A cut off level of 30 mg/L SAA level will be chosen to reflect a real increase in its level

Eligibility

Sex: FEMALEMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: 1. women with unexplained infertility (unprotected, regular intercourse for at least 2 years). 2. Nulligravida ,Regular mensturation. 3. Age less than 40 years . 4. Normal parameters (in the study group): * hormonal profile (day 2 FSH,LH), midluteal phase progesterone, Prolactin, TSH * transvaginal ultrasound * hysterosalpingography * detailed semen analysis Exclusion Criteria: 1. Age above 40 2. Smoking females 3. Obesity (BMI\>30) 4. Women with any systemic or medical diseases leads to elevation of SAA as: 1. Systemic lupus erythematosis 2. Rheumatoid arthritis 3. Sarcoidosis 4. Diabetes Mellitus 5. Using hormonal contraception (for control group)

Locations (1)

Ain Shams University Hospital

Cairo, Egypt

Outcomes

Primary Outcomes

Difference between SAA level between cases and control.

Time frame: 1 day (once)

Secondary Outcomes

Correlation between SAA level with other parameters in the hormonal profile (e.g., FSH, LH, E2).

Time frame: 1 day (once)

Data from ClinicalTrials.gov

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