The aim of this study is to correlate and identify the biochemical markers which may reflect WOI. Blood samples will be collected and endometrial biopsy will be performed at particular time points.
Endometrial receptivity in the midluteal phase of the menstrual cycle, known as the window of implantation (WOI), occurs only for a very short period of time. However, so far, there is no reliable clinical and biochemical indicators of WOI. Therefore, this study is to correlate and identify the biochemical markers which may reflect WOI. participants will be asked to be: 1. performed endometrial biopsy 2. taken blood for a few time points (e.g. pre-conception and being pregnant) and to compare different kinds of biochemical markers.
Study Type
OBSERVATIONAL
Enrollment
220
patient will be performed endometrial biopsy and collected blood samples at particular time points
Chinese University of Hong Kong
Hong Kong, Hong Kong
RECRUITINGThe change of the transcriptome profiling in the peripheral blood
RNA per sample will be prepared into two portions. One is for RNA sequencing and the other is for small RNA sequencing. The next step is hydrolysis of RNA into 200-300 nucleotides prior to reverse transcription. When sequencing RNA other than mRNA the library preparation is modified. The cellular RNA is selected based on the desired size range. For miRNA, the RNA is isolated through size selection. Once isolated, linkers are added to the 3' and 5' end then purified. The final step is cDNA generation through reverse transcription.
Time frame: From the day of endometrial biopsy through study completion, an average of 2 months
The change of the immune markers in the peripheral blood
Immune markers including cytokines such as leukemia inhibitory factor, macrophage colony stimulating factor, interleukin - 1 beta, transforming growth factor beta-1, Tim-3, galectin-9 and other markers considered to play a role in implantation will also be measured.
Time frame: From the day of endometrial biopsy through study completion, an average of 2 months
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