The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males

Inclusion Criteria: * Male * 18-40 years of age * Body mass index between 18.5 and 30 Exclusion Criteria: * Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) * Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension * Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism) * A personal or family history of epilepsy, seizures or schizophrenia * Any known disorders in muscle metabolism * Regular use of nutritional supplements * Allergy to lidocaine * Allergy to one or multiple amino acids * Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches. * Having received or ingested a stable isotope tracer in the past