To explore the association among gene, TCM pattern, TCM tongue diagnosis and TCM pulse diagnosis with the DNA Exome sequencing tools for Sjögren's syndrome
Objective: To explore the association among gene, TCM pattern, TCM tongue diagnosis and TCM pulse diagnosis with the DNA Exome sequencing tools for Sjögren's syndrome Method: This study wants to establish the reference value of DNA gene variants of healthy control for comparing with the Sjögren's syndrome subjects. And the investigators want to use the DNA Exome sequencing tools, proteomics and oral microbiome to explore the association of gene expression between at least 30 Sjögren's syndrome subjects and at least 10 healthy control subjects. Finally, the investigators also analyze the association among gene expression, proteomics and oral microbiome, TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, and heart rate variability (HRV) for this purpose. Expected Results: 1. To evaluate the difference among TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, and HRV for the Sjögren's syndrome and healthy control. 2. To evaluate the immunity-related gene expression for the Sjögren's syndrome and healthy control. 3. To evaluate the proteomics for the Sjögren's syndrome and healthy control. 4. To evaluate the oral microbiome for the Sjögren's syndrome and healthy control. 5. To evaluate the association among the immunity-related gene expression, SSA, SSB and RF. Keyword: Sjögren's syndrome, Immune regulation, Gene modulation, TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, proteomics, oral microbiome
Study Type
OBSERVATIONAL
Enrollment
40
The DNA of Sjögren's syndrome patients and health subjects will take to do the DNA Exome sequencing.
The saliva of Sjögren's syndrome patients and health subjects will take to do the oral microbiome.
The protein of Sjögren's syndrome patients and health subjects will take to do the proteomics.
Ching-Mao Chang
Taipei, Taiwan (r.o.c.), Taiwan
Immunity-related gene expression
To evaluate the immunity-related gene expression for the Sjögren's syndrome and healthy control. DNA exome combine the GATK (Genome Analysis Toolkit) is a tool for detecting the ratio of immunity-related gene expression.
Time frame: One year
TCM pattern
To evaluate the difference of TCM pattern between Sjögren's syndrome and healthy control. TCM pattern is a tool for detecting the constitution.
Time frame: One year
TCM tongue diagnosis
To evaluate the difference of TCM tongue diagnosis between Sjögren's syndrome and healthy control. TCM tongue diagnosis is a tool for detecting tongue expression.
Time frame: One year
TCM pulse diagnosis
To evaluate the difference of TCM pulse diagnosis between Sjögren's syndrome and healthy control. TCM pulse diagnosis is a tool for detecting pulse of radial artery.
Time frame: One year
Heart rate variability (HRV)
To evaluate the difference of Heart rate variability (HRV) between Sjögren's syndrome and healthy control. HRV is a tool for detecting the ratio between High-frequency (HF) activity and low-frequency (LF) activity.
Time frame: One year
Immunity-related proteomics expression
To evaluate the proteomics for the Sjögren's syndrome and healthy control. Proteomics is a tool for detecting the ratio of immunity-related proteomics expression.
Time frame: One year
Immunity-related microbiome expression
To evaluate the oral microbiome for the Sjögren's syndrome and healthy control. Oral microbiome is a tool for detecting the ratio of immunity-related microbiome expression.
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The RNA of Sjögren's syndrome patients and health subjects will store to do the RNA sequencing.
The serum of Sjögren's syndrome patients and health subjects will store to do the cytokine detection in the future.
Sjögren's syndrome patients and health subjects will take the Traditional Chinese Medicine pattern.
Sjögren's syndrome patients and health subjects will take the Traditional Chinese Medicine tongue diagnosis.
Sjögren's syndrome patients and health subjects will take the Traditional Chinese Medicine pulse diagnosis.
Sjögren's syndrome patients and health subjects will take the heart rate variability.
Sjögren's syndrome patients will take the Schirmer's test.
Sjögren's syndrome patients and health subjects will take the blood function test (WBC, RBC, Hb, HCT, Platelet, MCV, MCH, and MCHC), liver function test (AST and ALT), kidney function test (BUN and Cre), and immune function test (RF, Anti-SSA, Anti-SSB Ab, ESR, and CRP)
Time frame: One year