Clinical Research of ROBO1 Specific CAR-NK Cells on Patients With Solid Tumors

Inclusion Criteria: 1. Age 18 \~ 75 years old, male or female 2. Life expectancy ≥ 6 months 3. ECOG score: 0 - 3 4. ROBO1 expression in malignancy tissues detected by immuno-histochemistry (IHC) 5. Advanced solid tumor was diagnosed by pathological or clinical physicians 6. Laboratory examination: white blood cell ≥ 3 x 10\*9/L, blood platelet count ≥ 60 x 10\*/L, hemoglobin ≥ 85g/L; lymphocyte count ≥ 15%, total bilirubin ≤ 100 mol/L; ALT and AST less than five times of the normal level; serum creatinine less than 1.5 times of the normal level 7. Signed informed consent 8. Women of child-bearing age must have evidence of negative pregnancy test and be willing to practice birth control after 2 weeks following the cell transfusion Exclusion Criteria: 1. Expected overall survival \< 6 months 2. Patients with uncontrolled hypertension, unstable coronary disease (uncontrolled arrhythmias, unstable angina, decompensated congestive heart failure (\> Class II, NYHA), or myocardial infarction within 6 months 3. Abnormal lung function: FEV (forced expiratory volume) \< 30% prediction, DLCO (diffusing capacity of the lung for carbon monoxide) \< 30% prediction, blood oxygen saturation \< 90% 4. Other serious diseases: nervous, mental disorders, immune regulatory diseases, metabolic diseases, infectious diseases, etc. 5. Unable or unwilling to provide informed consent, or fail to comply with the test requirements