Allogenic HSCT brings significant changes in biodiversity and composition of the gut microbiome through antibiotic usage, the mucosal damage due to the chemo- and radiotherapy toxicity; compromised oral nutritional intake and graft-versus-host disease with gut damage as the complication. Aim of the study is to investigate the composition of the microbiota in both recipient and nursing relative donor, reveal changes in biodiversity after HSCT via 3-time points V3V4 16S rRNA and NGS sequencing of the colon and oral swabs, 3-indoxyl-sulfate measurement in the urine.
Study Type
OBSERVATIONAL
Enrollment
15
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology
Moscow, Russia
beta-index dynamics
biodiversity (beta-index) dynamics between time points before and after HSCT
Time frame: 7 days before HSCT
beta-index dynamics
biodiversity (beta-index) dynamics between time points before and after HSCT
Time frame: 7 days after HSCT
beta-index dynamics
biodiversity (beta-index) dynamics between time points before and after HSCT
Time frame: 60 days after HSCT
Proportion of patients with microbial domination after HSCT
evaluation of the dynamics of the microbial domination between time points before and after HSCT
Time frame: 7 days after HSCT
Proportion of patients with microbial domination after HSCT
evaluation of the dynamics of the microbial domination between time points before and after HSCT
Time frame: 60 days after HSCT
Shannon-index dynamics
biodiversity (Shannon-index - an information statistic index, which means it assumes all species are represented in a sample and that they are randomly sampled) dynamics between time points before and after HSCT
Time frame: 7 days before HSCT
Shannon-index dynamics
biodiversity (Shannon-index - an information statistic index, which means it assumes all species are represented in a sample and that they are randomly sampled) dynamics between time points before and after HSCT
Time frame: 7 days after HSCT
Shannon-index dynamics
biodiversity (Shannon-index - an information statistic index, which means it assumes all species are represented in a sample and that they are randomly sampled) dynamics between time points before and after HSCT
Time frame: 60 days after HSCT
ROC (receiver operating characteristic)-curve of NGS (new-generation sequence)
sensitivity of NGS as a method of biodiversity evaluation
Time frame: 7 days before HSCT
ROC (receiver operating characteristic)-curve of NGS (new-generation sequence)
sensitivity of NGS as a method of biodiversity evaluation
Time frame: 7 days after HSCT
ROC (receiver operating characteristic)-curve of NGS (new-generation sequence)
sensitivity of NGS as a method of biodiversity evaluation
Time frame: 60 days after HSCT
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