The purpose of this study is to examine and compare the effects of autogenous dental pulp tissue on bone formation in the extraction sockets as compared to commonly used particulate bone graft. The effects on bone formation will be examined using a wide variety of assays.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Creos allo.gain allogenic bone particulate mineralized cortical bone will be hydrated and placed in the extraction socket.
Dental pulp will be isolated from teeth extracted for non-periodontal reasons chairside, mixed with hydrated particulate bone graft, and then placed in the extraction socket.
After placement of particulate bone graft or particulate bone graft plus autogenous dental pulp tissue, socket will be covered by a resorbable collagen membrane and sutured.
The University of Texas Health Science Center at Houston
Houston, Texas, United States
Bone fill as assessed by radiograph
Time frame: immediately after placement of bone graft
Bone fill as assessed by radiograph
Time frame: 2 months after placement of bone graft
Bone fill as assessed by radiograph
Time frame: 4 months after placement of bone graft
Extent of mineralization as assessed by von Kossa staining
Time frame: 4 months after placement of bone graft
Extent of mineralization as assessed by Xylenol Orange staining
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker Bsp assessed by quantitative PCR (qPCR)
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker BSP assessed by immunostaining using anti-BSP antibody
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker Bglap assessed by quantitative PCR (qPCR)
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker BGLAP assessed by immunostaining using anti-BGLAP antibody
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker Dmp1 assessed by quantitative PCR (qPCR)
Time frame: 4 months after placement of bone graft
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Resorbable or non-resorbable suture material
Expression of osteoblastic marker DMP1 assessed by immunostaining using anti-DMP1 antibody
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker Col1a1 assessed by quantitative PCR (qPCR)
Time frame: 4 months after placement of bone graft
Expression of osteoblastic marker Sost assessed by quantitative PCR (qPCR)
Time frame: 4 months after placement of bone graft