Red meat is an integral component of the habitual diet among the UK and Irish population, with adults consuming an average of 71grams/day. Although typically high in saturated fatty acids (SFA), red meat is also an important dietary source of protein and essential nutrients including iron, zinc, B vitamins and long chain n-3 polyunsaturated fatty acids (PUFA) which provide numerous benefits to human health, particularly related to cardiovascular disease (CVD) risk. N-3 and n-6 PUFA are a family of fatty acids with important roles in cardiovascular health, and it is often recommended in dietary guidelines to replace SFA with unsaturated fats, such as PUFA. Owing to the social and economic burden of CVD, increasing the proportions of these unsaturated fatty acids, in combination with a reduction in SFA within meat, could have a large impact on CVD risk at the population level, whilst retaining the beneficial nutrients and n-3 PUFA which meat provides. In this research, a total of 90 eligible and consenting participants will be randomly allocated to consume three portions per week of n-3 enriched beef (from either dietary supplemented or grass-fed cattle) or control beef (from standard supply). This beef will be offered within a lunchtime meal and served from the Human Intervention Studies Unit at Ulster University, Coleraine for a period of 5 weeks. A fasting blood sample will be taken before and after intervention to determine the effect the n-3 enriched beef on cholesterol concentrations, lipid profile, PUFA status and inflammation. Blood pressure, stiffness of the arteries and body shape, size and composition will also be assessed, and some health and lifestyle habits will be captured using questionnaires.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
TRIPLE
Enrollment
90
Lunches served in Human Intervention Studies Unit
Human Intervention Studies Unit, Ulster University
Coleraine, Co.Londonderry, United Kingdom
Cholesterol levels
Plasma cholesterol measured colormetrically
Time frame: Change at 5 weeks from baseline
Lipid profile
Plasma lipids measured colormetrically
Time frame: Change at 5 weeks from baseline
Polyunsaturated fatty acid profile
Serum fatty acids measured by GC-MS
Time frame: Change at 5 weeks from baseline
Pulse Wave Velocity
Measured by Sphygmocor XCEL
Time frame: Change at 5 weeks from baseline
Blood pressure
Measured by electronic blood pressure monitor
Time frame: Change at 5 weeks from baseline
Whole body composition
Measured by Dual Energy X-ray Absorptiometry
Time frame: Change at 5 weeks from baseline
Inflammation status
Measured by ELISA
Time frame: Change at 5 weeks from baseline
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