Automated immunodosage methods (Roche Elecsys cortisol and IDS cortisol dosing kits) offer a simple and inexpensive technology routinely used in a medical biology laboratory. They can be used to define robust diagnostic thresholds for salivary cortisol for the diagnosis of Cushing's syndrome and pseudo-Cushing combining the three tests performed as part of the patient's usual management. (ie two urinary free cortisol (UFC), the dexamethasone suppression test, and Diurnal variation of plasma cortisol).
Study Type
OBSERVATIONAL
Enrollment
380
Plasma cortisol level test
Urinary free cortisol test
salivette® for salivary cortisol quantification
Dectancyl® 2 x 0.5 mg tablets (total 1 mg)
CHU de Nimes
Nîmes, France
Salivary cortisol measured by Roche Elecsys cortisol kit
nmol/l
Time frame: Day 1 (at screening)
Salivary cortisol measured by IDS cortisol dosing kit at screening
nmol/l
Time frame: Day 1 (at screening)
Salivary cortisol measured by Roche Elecsys cortisol kit
nmol/l
Time frame: Day 2 and Day 3
Salivary cortisol measured by IDS cortisol dosing kit
nmol/l
Time frame: Day 2 and Day 3
Salivary cortisol measured measured by LC-MS/MS
nmol/l
Time frame: Day 1, 2 and 3
Dexamethasone suppression test
µg/l and nmol/l (cut-off for diagnosis: 50nmol/l)
Time frame: Day 4
Diurnal variation of plasma cortisol
Roche Elecsys cortisol kiet; µg/l and nmol/l (cut-off for diagnosis: 200nmol/l)
Time frame: Day 3
Urinary free cortisol
Radioimmunoassay; µg/l and nmol/l
Time frame: Day 3
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.