The study aims to investigate the inflammatory response of endothelial cells to various stimulations, in particular the production of IL33 and of endocan in response to allergens, agonists of microorganisms and pollutants. For that purpose, this project attempts to set up a biological collection of lung and umbilical cord endothelial cells. Lung endothelial cells are resected from a surgical specimen, resulting from a lung cancer surgery. Umbilical cord-derived endothelial cells are taken from the umbilical cord collected during the delivery.
Study Type
OBSERVATIONAL
Hop Calmette Chu Lille
Lille, France
change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.
Time frame: change from Baseline at 48 hours after stimulation
change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours after stimulation
change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation
change in IL33 protein concentration between stimulated and unstimulated primary human lung endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation
change in IL33 protein concentration between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation
change in the level of messenger RNA expressed as endocan / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation
change in the level of messenger RNA expressed as endocan / GAPDH ratio between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation
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change in endocan protein concentration between stimulated and unstimulated primary human lung endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation
change in endocan protein concentration between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.
Time frame: change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation