The purpose is to examine the effects of time-restricted eating (TRE; consuming all calories within an 8-hour period each day) vs. normal eating (CON; consuming same kcals and protein as TRE, but during a 10-13 hr eating window each day) during 8 weeks of resistance exercise on body composition and muscle mass (whole muscle and single fiber), muscular performance, anabolic protein signaling, single muscle fiber characteristics, and the gut microbiome in well-trained young men and women.
The primary goal pertains to muscle hypertrophy (not fat loss), thus all participants will be placed in a 10% caloric surplus.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
DOUBLE
Enrollment
20
Diet will be tracked and reported every day to a qualified dietitian.
Diet will be tracked and reported every day to a qualified dietitian.
8 weeks of supervised and programmed whole body resistance training, performed 4x per week
Center for Sport Performance at California State University, Fullerton
Fullerton, California, United States
Changes in Muscle Size - Lower Body
via Ultrasound
Time frame: Pre, Mid (4 weeks), and Post (8 weeks)
Changes in Muscle Size - Upper Body
via Ultrasound
Time frame: Pre, Mid (4 weeks), and Post (8 weeks)
Changes in Fiber Type-Specific Size
Single muscle fiber analysis
Time frame: Pre and Post (week 8)
Changes in Body composition
via BodPod
Time frame: Pre, Mid (4 weeks), and Post (8 weeks)
Acute Exercise Induced Fiber Type-Specific Anabolic Signaling (Total Concentration and Phosphorylation Status)
Men only; assessed via single fiber Simple Western
Time frame: Day 1 of exercise training
Changes in Maximal Strength
1RM on bench press, squat, and leg extension
Time frame: Pre and Post (week 8)
Changes in Maximal Muscular Endurance
Maximal repetitions on bench press and leg press
Time frame: Pre and Post (week 8)
Changes in Gut Microbiome
via stool sample
Time frame: Pre, Mid (week 2, 4, 6), and Post (week 8)
Changes in Fiber Type Composition
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Consumed on all exercise days.
Single Fiber via SDS-PAGE
Time frame: Pre and Post (week 8)
Changes in Fiber Type-Specific Resting Anabolic Signaling (Total Concentration and Phosphorylation Status)
via single fiber Simple Western
Time frame: Pre and Post (week 8)
Changes in Mood
via Questionnaire
Time frame: Weekly for duration of the intervention (8 weeks)
Changes in Enjoyment of Diet
via Questionnaire
Time frame: Weekly for duration of the intervention (8 weeks)
Changes in Sleep (total and quality)
via Questionnaire
Time frame: Weekly for duration of the intervention (8 weeks)
Changes in Menstrual Cycle
via Questionnaire; women only
Time frame: Pre, Mid (week 4), and post (week 8)
Total Training Volume
Time frame: After 8 weeks
Nutrient Intake
Total Kcal and grams of protein, fat, and carbohydrates
Time frame: Daily, starting 7 days before the study and continuing for 8 additional weeks