Worldwide, colorectal cancer is the 3rd most common cancer; risk increases with age and is modified by lifestyle factors notably diet, physical activity and obesity. The BORICC Follow-Up (BFU) Study is a 12+ year follow-up of participants recruited to the Biomarkers of Risk of Colon Cancer (BORICC) Study. This longitudinal study will investigate associations between ageing and lifestyle factors and a panel of molecular biomarkers linked with colorectal cancer risk.
The BORICC Follow-Up (BFU) Study builds on the findings from the BORICC Study where the investigators observed associations between age and nutritional factors, including selenium and folate, and biomarkers of colorectal health. The BFU Study will investigate the relationships between ageing (12+ years) and such biomarkers longitudinally. It is anticipated that the project will produce novel data on (i) changes in biomarkers of colorectal cancer risk with age and (ii) the effects of obesity and lifestyle factors on biomarkers of colorectal cancer risk. These biomarkers include differentially expressed proteins, DNA methylation markers and inflammation markers.
Study Type
OBSERVATIONAL
Enrollment
47
North Tyneside General Hospital
North Shields, Tyne & Wear, United Kingdom
Faecal calprotectin concentrations (marker of local inflammation)
Time frame: 12 years (on average)
Serum high-sensitivity C-Reactive Protein concentrations (marker of systemic inflammation)
Time frame: 12 years (on average)
Gut microbiota (analysed in stool samples)
Abundance and diversity of the gut microbiota assessed in stool samples
Time frame: 12 years (on average)
Faecal short-chain fatty acid concentrations
Concentrations and proportions of short-chain fatty acids e.g. acetate, propionate and butyrate
Time frame: 12 years (on average)
DNA methylation in rectal mucosal biopsies(% methylation)
Target gene and global methylation (LINE-1) in rectal mucosal biopsies
Time frame: 12 years (on average)
Target gene expression in rectal mucosal biopsies
Expression of genes related to inflammation and the WNT signalling pathway in rectal mucosal biopsies
Time frame: 12 years (on average)
microRNA expression in rectal mucosal biopsies
Expression of microRNAs in rectal mucosal biopsies
Time frame: 12 years (on average)
Colonic crypt cell proliferative state
Total number and distribution of proliferating cells in rectal mucosal crypts
Time frame: 12 years (on average)
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Colonic crypt cell dimensions
Height (length) and width rectal mucosal crypts
Time frame: 12 years (on average)
Markers of mitochondrial function and structure in rectal mucosal biopsies
Markers of mitochondrial function and structure in rectal mucosal biopsies such as oxidative phosphorylation proteins, namely complex I and IV
Time frame: 12 years (on average)
Parathyroid hormone in plasma
Time frame: 12 years (on average)
25-hydroxy vitamin D concentrations in serum
Time frame: 12 years (on average)
Vitamin B12 concentrations in serum
Time frame: 12 years (on average)
Folate concentrations in serum
Time frame: 12 years (on average)
Triglycerides in plasma
Time frame: 12 years (on average)
HDL cholesterol in plasma
Time frame: 12 years (on average)
LDL cholesterol in plasma
Time frame: 12 years (on average)
Total cholesterol in plasma
Time frame: 12 years (on average)
Glucose in plasma
Time frame: 12 years (on average)
HbA1c in whole blood
Time frame: 12 years (on average)