The study aims at the identification and the quantification of urinary branched-chain amino acids (BCAAs) by mass spectometry in healthy subjects and patients with insulin resistance and correlating urinary BCAAs with HOMA index. Blood and urine levels of BCAAs will be correlated with HOMA index to assess and quantify insulin resistance. Identification and quantification of urine BCAAs by an innovative method based on synthetic biology will also be performed. Correlations between mass spectometry and the innovative detection method of BCAAs will be searched.
Blood and urine samples will be collected in 330 persons to assess BCAAs levels and correlate them with HOMA index. Persons included will be: 110 persons with BMI\>27 and HOMA \>4 with no diabetes 110 persons with BMI\>27 and HOMA \< 4 with no diabetes 110 persons with BMI \< 27 and HOMA \<4 with no diabetes, including at least 50 men and 50 women, 30 aged 18-35, 30 aged 36-55, 30 aged 56-75. Urine BCAAs will be measured by both mass spectrometry and the innovative method based on synthetic biology.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
304
blood and urine sample collection for assays of BCAAs
UH Montpellier
Montpellier, France
Correlation between urinary levels of BCAAs and HOMA index
Identification of urinary BCAAs as markers of insulin resistance
Time frame: through study completion, an average of two years and a half
Correlation between blood and urine BCAAs levels
Validation of urinary BCAAs as biomarkers of insulin resistance
Time frame: through study completion, an average of two years and a half
Correlation between urine BCAAs levels measured by enzymatic method (artificial vesicles) and classic method
Measured by enzymatic method (artificial vesicles) and classic method (standard LC-MS/MS)
Time frame: through study completion, an average of two years and a half
Correlation between urine BCAAs levels measured by enzymatic method (artificial vesicles) and HOMA index
Measured by enzymatic method (artificial vesicles) and HOMA index
Time frame: through study completion, an average of two years and a half
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