Possible Role of Gingival Crevicular Fluid Levels of Chemerin and Fibroblast Growth Factor 21 as Biomarkers of Periodontal Disease in Diabetic and Non-diabetic Patients

Inclusion Criteria: * age range between 35 and 60 years * a minimum of 20 natural teeth. * Type 2DM patients exhibiting HbA1c 6-8% and FPG ≥126 mg/dL for more than one year who did not complain of any systemic diseases other than t2DM * periodontitis patients with moderate to severe periodontitis having gingival index GI ≥ 1, probing depth PD ≥ 5, and clinical attachment level CAL ≥ 4 mm, and bone loss affecting \> 30% of existing teeth on clinical/radiographic examination Exclusion criteria * uncontrolled diabetes mellitus * systemic diseases which may affect the biomarkers levels and the periodontal conditions. * Exposure to steroid therapies, radiation/immune-suppressive therapies. * Allergic reaction to any kind of drug. * Smoking over the past 5 years. * Periapical pathologies. * Exposure to mechanical forces as a result of occlusion/ orthodontics. * History of periodontal or drug therapies within the previous 6 months.