Clear cell renal cell carcinoma is diagnosed by imaging, sometimes associated with biopsy. This diagnosis is expensive, invasive and sometimes late. The development of a simple biological test for diagnosis is essential. Exosomes are 30 to 150 nm membrane vesicles secreted into the extracellular space by various living cells. These exosomes can be isolated from biological fluids, including urine. The recent study of urinary exosomes is a promising topic for analyzing tumor markers in urine. The investigator's goal is to develop a reliable technique for detecting tumor exosomes in urine in patients with clear cell renal cell carcinoma. The analysis of urinary exosomes could provide a new liquid biopsy tool for the early diagnosis of clear cell renal cell carcinoma.
The primary objective is to detect urinary exosomes, by electron microscopy technique, from clear cell renal cell carcinoma due to exosome markers (CD9) and clear cell kidney cancer (CA9).
Study Type
OBSERVATIONAL
Enrollment
100
Urinary sample (100 ml) will be collected in the urology department to analysis urinary exosomes.
Chu Saint-Etienne
Saint-Etienne, France
RECRUITINGNumber of patients (with clear cell kidney cancer) with CD9+ and CA9+ exosomes by electron microscopy technique
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+ /CA9+ exosomes by electron microscopy technique (%) for patients with clear cell kidney cancer
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+ /CA9+ exosomes by electron microscopy technique (%) for control patients
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+ /CA9+ exosomes by flow cytometry (%) for patients with clear cell kidney cancer
Measured by analysis in flow cytometry of urinary sample.
Time frame: At inclusion
Percentage of CD9+ /CA9+ exosomes by flow cytometry (%) for control patients
Measured by analysis in flow cytometry of urinary sample.
Time frame: At inclusion
Percentage of CD9+ /CA9+ exosomes by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) (%) for control patients
Measured by analysis in Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) of urinary sample.
Time frame: At inclusion
Percentage of CD9+ /CA9+ exosomes by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) (%) for patients with clear cell kidney cancer
Measured by analysis in Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) of urinary sample.
Time frame: At inclusion
Percentage of CD9+/CD63+/CD81+/CA9+ exosomes by electron microscopy technique (%) for patients with clear cell kidney cancer
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+/CD63+/CD81+/CA9+ exosomes by electron microscopy technique (%) for control patients
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+/CD63+/CD81+/CA9+ exosomes by flow cytometry (%) for patients with clear cell kidney cancer
Measured by analysis in flow cytometry of urinary sample.
Time frame: At inclusion
Percentage of CD9+/CD63+/CD81+/CA9+ exosomes by flow cytometry (%) for control patients
Measured by analysis in flow cytometry of urinary sample.
Time frame: At inclusion
Percentage of CD9+/CD63+/CD81+/CA9+ exosomes by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) (%) for patients with clear cell kidney cancer
Measured by analysis in Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) of urinary sample.
Time frame: Years: 2
Percentage of CD9+/CD63+/CD81+/CA9+ exosomes by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) (%) for control patients
Measured by analysis in Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) of urinary sample.
Time frame: Years: 2
Percentage of CD9+/VGEFR2+ exosomes by electron microscopy technique (%) for patients with clear cell kidney cancer
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+/VGEFR2+ exosomes by electron microscopy technique (%) for control patients
Measured by analysis in electron microscopy technique of urinary sample.
Time frame: At inclusion
Percentage of CD9+/VGEFR2+ exosomes by flow cytometry (%) for patients with clear cell kidney cancer
Measured by analysis in flow cytometry of urinary sample.
Time frame: At inclusion
Percentage of CD9+/VGEFR2+ exosomes by flow cytometry (%) for control patients
Measured by analysis in flow cytometry of urinary sample.
Time frame: At inclusion
Percentage of CD9+/VGEFR2+ exosomes by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) (%) for patients with clear cell kidney cancer
Measured by analysis in Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) of urinary sample.
Time frame: At inclusion
Percentage of CD9+/VGEFR2+ exosomes by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) (%) for control patients
Measured by analysis in Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) of urinary sample.
Time frame: At inclusion
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