Acute Effect of modeRate-intensity aerOBIc Exercise on Colon Cancer Cell Growth

Northumbria University16 enrolled

Overview

This study involves drawing blood samples from men before and after they perform 30-minutes of moderate-intensity aerobic exercise. The investigators will evaluate whether adding the exercise serum to colon cancer cells in a dish can reduce the growth of the cells compared to the resting serum. Note: serum is the liquid part of the blood that carries hormones and metabolites around the body.

Regular exercise is associated with a reduced risk of developing colon cancer. However, the mechanisms underpinning the anti-cancer effect of exercise are not yet fully understood. A recent theory suggests that each time you exercise, the short-lasting spikes in circulating hormones can suppress the growth of cancer cells. Hence, every exercise bout could have a direct anti-cancer effect. This study will recruit men with an increased of colon cancer and explore whether incubating colon cancer cells with serum collected after a bout of moderate-intensity aerobic exercise influences cell viability in vitro.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

NONE

Enrollment

Conditions

Colon Adenocarcinoma Colon Cancer

Interventions

Exercise assessmentOTHER

The moderate-intensity aerobic interval exercise will be performed on a cycle ergometer under the supervision of trained staff in an exercise science laboratory. Participants will perform a 5 to 10-minute warm-up that begins by pedalling against a light resistance (60 W) and progressively increases in resistance until a target heart rate of 50-60% heart rate reserve is achieved. Participants will then complete 6 x 5-minute bouts at 60% heart rate reserve whilst maintaining a cadence of 60 rev·min-1, separated by 2.5-minutes of pedalling against light resistance (60 W). The session will finish with a cool-down at light resistance (60 W) lasting 10-minutes.

Eligibility

Sex: MALEMin age: 50 YearsMax age: 90 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Age ≥ 50 years * BMI ≥ 25 kg/m2 and/or waist circumference of ≥ 94 cm * Male * Participating in less than 30 min of planned, structured, moderate to vigorous-intensity physical activity on three or more d·wk-1 for the last three months Exclusion Criteria: * Any absolute or relative contraindication to exercise testing, as determined by the American College of Sports Medicine * Any sign/symptom of cardiovascular, metabolic or renal disease * Known cardiovascular, metabolic or renal disease without written medical clearance from physician * Resting hypertension (≥160 mmHg systolic and/or ≥90 mmHg diastolic) * Chronic obstructive pulmonary disease and/or asthma with peak respiratory flow \< 300 l/min * Previous stroke or transient ischemic attack * Epilepsy or aneurysm (large vessel or cerebral) * Previous or current treatment for malignancy * Clotting disorder * Taking beta-adrenergic blocking agents * Resting heart rate ≥ 100 bpm * Musculoskeletal, neurological, anthropometric, or rheumatoid conditions that makes it not possible to pedal a bicycle and/or would be worsened due to exercise * Body mass \> 150 kg

Locations (2)

York St John University Sports Park

York, North Yorkshire, United Kingdom

Northumbria University City Campus

Newcastle upon Tyne, United Kingdom

Outcomes

Primary Outcomes

Cell viability (%)

Viability of a human colon cancer cell line (LoVo) will be assessed via quantification of the fluorescent signal by the resazurin assay (Sigma-Aldrich, Dorset, UK). Cells will be seeded in culture medium containing 10% serum from individual participants and incubated for 48 hours. Fluorescence will then be measured using a microplate reader at an excitation of 540 nm and emission of 590 nm. Background fluorescence will be subtracted from each well and then values will be normalised to fluorescence of control cells grown in 10% fetal bovine serum instead of participant serum to give a percent viability.

Time frame: 48 hours

Secondary Outcomes

Concentration of epinephrine (pg/ml)

Systemic concentration of epinephrine will be measured from serum

Time frame: Immediately before and immediately after the exercise and resting assessments

Concentration of norepinephrine (pg/ml)

Systemic concentration of norepinephrine will be measured from serum

Time frame: Immediately before and immediately after the exercise and resting assessments

Concentration of Interleukin 6 (pg/ml)

Systemic concentration of Interleukin 6 will be measured from serum

Time frame: Immediately before and immediately after the exercise and resting assessments

Concentration of Tumour Necrosis Factor alpha (pg/ml)

Systemic concentration of Tumour Necrosis Factor alpha will be measured from serum

Time frame: Immediately before and immediately after the exercise and resting assessments

Concentration of Insulin (pmol/l)

Systemic concentration of Insulin will be measured from serum

Data from ClinicalTrials.gov

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