The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.
400 people (250 - study group; 150 control group) were subjected to subjective and objective action. Information on the occurrence of diseases and cardiovascular risk in the participant and his family was collected from research studies. Anthropometric parameters were measured (body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure and pulse measurement. In addition, fasting venous blood was collected and secured. In venous blood currently marked with the following concentration: glucose, required cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and protecting DNA was developed from blood. Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR (High Resolution Melt) method was determined. Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.
Study Type
OBSERVATIONAL
Enrollment
400
determination of VEGF gene expression, VEGF gene polymorphism and serum leptin concentration
Department of Treatment of Obesity, Metabolic Disorders and Clinical Dietetics, Poznan University of Medical Sciences
Poznan, Wielkopolska, Poland
VEGF gene expression
VEGF gene expression
Time frame: through study completion, an average of 1 year
VEGF gene polymorphism
VEGF gene polymorphism
Time frame: through study completion, an average of 1 year
serum leptin concentration
serum leptin concentration
Time frame: through study completion, an average of 1 year
Body mass (BM)
Body mass
Time frame: through study completion, an average of 1 year
Waist circumference (WC)
Waist circumference
Time frame: through study completion, an average of 1 year
total cholesterol (TCH)
Blood concentration of total cholesterol
Time frame: through study completion, an average of 1 year
low density lipoprotein (LDL)
Blood concentration of low density lipoprotein
Time frame: through study completion, an average of 1 year
high density lipoprotein (HDL)
Blood concentration of high density lipoprotein
Time frame: through study completion, an average of 1 year
triglycerides
Blood concentration of triglycerides
Time frame: through study completion, an average of 1 year
glucose
Blood concentration of glucose
Time frame: through study completion, an average of 1 year
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