In this four-way crossover study, a plasmakinetic profile of GABA from tomatoes as compared to a gamma-aminobutyric acid (GABA) supplement in healthy young men will be established. In addition, a plasmakinetic profiles of glutamate from a supplement and from tomatoes as well as the effects of glutamate from tomatoes and a supplement on the GABA plasma levels will be determined. This study will therefore provide information about the effect of a food matrix on GABA plasmakinetics.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
12
A maximum of 1 kilo pureed tomatoes, containing 1 gram of GABA.
1 gram of GABA dissolved in water.
A maximum of 8 grams glutamate, adjusted to amount of glutamate given in the tomato arm. Dissolved in water.
Wageningen University, Division of Human Nutrition
Wageningen, Netherlands
Area under the plasma concentration versus time curve (AUC ) of plasma-time curves of GABA
After intake of a GABA supplement and tomatoes.
Time frame: 6 months
Peak Plasma Concentration (Cmax) of plasma-time curves of GABA
After intake of a GABA supplement and tomatoes.
Time frame: 6 months
Time to Peak Plasma Concentration (Tmax) of plasma-time curves of GABA
After intake of a GABA supplement and tomatoes.
Time frame: 6 months
Half-life (T1/2) of plasma-time curves of GABA
After intake of a GABA supplement and tomatoes.
Time frame: 6 months
Area under the plasma concentration versus time curve (AUC) of plasma-time curves of glutamate
After intake of a glutamate supplement and tomatoes
Time frame: 6 months
Peak Plasma Concentration (Cmax) of plasma-time curves of glutamate
After intake of a glutamate supplement and tomatoes
Time frame: 6 months
Time to Peak Plasma Concentration (Tmax) of plasma-time curves of glutamate
After intake of a glutamate supplement and tomatoes
Time frame: 6 months
Half-life (T1/2) of plasma-time curves of glutamate
After intake of a glutamate supplement and tomatoes
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Time frame: 6 months
Area under the plasma concentration versus time curve (AUC) AUC of plasma-time curves of GABA in response to a glutamate supplement.
This will be compared to the plasma time-curves of GABA after tomato intake.
Time frame: 6 months
Peak Plasma Concentration (Cmax) of plasma-time curves of GABA in response to a glutamate supplement.
This will be compared to the plasma time-curves of GABA after tomato intake.
Time frame: 6 months
Time to Peak Plasma Concentration (Tmax) of plasma-time curves of GABA in response to a glutamate supplement.
This will be compared to the plasma time-curves of GABA after tomato intake.
Time frame: 6 months
Half-life (T1/2) of plasma-time curves of GABA in response to a glutamate supplement.
This will be compared to the plasma time-curves of GABA after tomato intake.
Time frame: 6 months