Investigation of the Gut Microbiome and Statin Response

Inclusion Criteria: 1. Participant is capable of giving informed consent 2. Participant is aged 18 to 65 years. The gut microbiome has been shown to change gradually with time, although there is no cut-off in age when this occurs. Exclusion Criteria: 1. Participants with cardiovascular disease such as a history of heart failure (New York Heart Association class II-IV), myocardial infarction, stroke, coronary artery bypass graft, hypertension, and hyperlipidemia as these conditions are associated with altered gut microbiome composition.74 Hypertension is defined as blood pressure greater than 160/110 or on any anti-hypertensive medications. LDL-C \>190 mg/dl or \<100 mg/dl and triglycerides \> 400 mg/dl. 2. Participants with a history of cancer. 3. Kidney disease (serum creatinine \>1.5 mg/dl). 4. Liver dysfunction (alanine aminotransferase \> 2 times the upper limit of normal). 5. Diabetes mellitus (DM) - Diabetes itself may affect the gut microbiome although this has not been extensively studied. In addition to a prior diagnosis of diabetes mellitus other than that related to pregnancy, a fasting glucose level of greater than 125mg/dL will be used to exclude participation. 6. Clinical diagnosis of hypothyroidism 7. History of inflammatory disorders of the intestinal tract (i.e. IBD, celiac sprue). 8. Use of antibiotics in the prior 6 months. 9. Use of pre-, pro-, or synbiotics. 10. Chronic medication use (including over the counter medications and herbal supplements) with the exception of oral contraceptives. Since we are evaluating the impact of rosuvastatin on the gut microbiome we would like to exclude the potential impact of confounding medications. 11. Current smoker. The effect of smoking on the microbiome of the gut is unknown. 12. Known history of alcohol or substance abuse. 13. Body Mass Index (BMI) \<18.5 or \>30 kg/m2. Volunteers with BMI below normal will be excluded to prevent inclusion of subjects with a subclinical systemic disease that may influence the gut microbiome. Volunteers with moderate or severe obesity will be excluded as obesity may be associated with altered gut microbiome composition.31 14. Unable to abstain from consumption of illicit drugs during the study period. 15. Prior bowel resection surgery other than appendectomy. It is unknown how prior bowel resection surgery may influence the microbiome composition; hence we will exclude these participants. 16. Baseline bowel frequency less than every 2 days or greater than 3 times daily. Normal bowel frequency is every 3rd day to 3 times per day. Although unknown, stool frequency could be related to the microbiome composition. To avoid the need for use of antidiarrheal medications or laxatives, which themselves could alter the microbiome composition, these patients will be excluded. 17. Participant has experienced diarrhea within the two weeks prior to entry. Diarrhea is defined as a change in bowel habits with an increased frequency or loose stools such that the stool could not be lifted with a fork. 18. Vegans and Vegetarians. 19. Known intolerance to statin medications. 20. Unwilling to obtain from grapefruit containing foods or drinks. 21. Pregnant women. To avoid any risk to an unborn fetus from study drug exposure. 22. Refusal to use two medically accepted method of birth control while participating in the study, such as a barrier method, hormonal contraceptives, implanted birth control devices, permanent methods (such as a vasectomy), and/or abstinence. 23. Nursing mothers 24. Any condition that the investigator feels may limit the volunteer's ability to complete the study protocol.