Expression Profiles of Integrin αDβ2 on Neutrophils in Sepsis

Boston Children's Hospital75 enrolled

Overview

In this research study we want to learn more about the characteristics of neutrophils that are present in the blood and secretions from a breathing tube of patients with sepsis. Sepsis is a severe type of infection, affecting various parts of the body. Neutrophils are a type of white blood cell that are part of the body's immune system. Even though neutrophils are important in getting rid of germs, they also may be harmful to parts of the body by causing injury in the lungs in patients with sepsis. Neutrophils can change their character in sepsis. Because of this, it is important for doctors to know what kind of neutrophils are in the blood and secretions from the breathing tube of patients with sepsis so that they can work to develop therapies to prevent these cells from being harmful.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Sepsis

Interventions

SepsisOTHER

Sepsis patients in the ICU with a breathing tube

Eligibility

Medical Language ↔ Plain English

Case Inclusion Criteria: * sepsis patient in the ICU with a breathing tube Control Inclusion Criteria: * scheduled for an elective surgical procedure and need preoperative blood draw or will have an intravenous catheter placed for the purpose of their surgical procedure * Plan to have endotracheal tube for planned procedures. Exclusion Criteria: * On chronic immunosuppressive drugs such as chronic corticosteroid use, or preexisting immunodeficiency diseases (oncology patients, patients with transplantation)

Outcomes

Primary Outcomes

Neutrophil characteristics

measure and report β2 integrin expression on neutrophils from blood and tracheal aspirate between sepsis patients and non-sepsis patients in pediatric population

Time frame: One day