IRAPe, Irisin and IL-34 in Patients With Type 2 Diabetes

Damanhour University120 enrolled

Overview

Aim: This study aimed to evaluate the role of extracellular part of insulin regulated aminopeptidase (IRAPe), IL-34, Irisin, and Visfatin in the development of insulin resistance in patients with type 2 diabetes mellitus. Methods: parallel study enrolled 60 non-diabetic control subjects and 60 newly-diagnosed type 2 diabetics, matched for age, body mass index and sex ratio. Anthropometric parameters, blood glucose, fasting plasma insulin, insulin resistance index (HOMA-IR), IL34, Irisin, IRAPe, Visfatin and glycated hemoglobin were assessed. Conclusion: IL-34 and Visfatin levels were significantly increased, while both Irisin and IRAPe levels were significantly decreased in patients with type 2 diabetes. Theses aforementioned parameters were significantly correlated with insulin resistance. These findings indicate that, IL-34, Irisin and IRAPe may play a vital role in T2DM and in diabetes associated insulin resistance. Additionally, the investigator's findings proposed that, IRAPe may be a useful and direct marker for detection of insulin resistance state.

Study Type

OBSERVATIONAL

Enrollment

120

Conditions

Type2 Diabetes Mellitus

Interventions

Blood sampleDIAGNOSTIC_TEST

Ten ml of venous blood was collected from all participants in the early morning after an overnight 12 hour fast by venipuncture of the antecubital vein into sterile tubes. Plasma and sera were separated immediately and were stored at -70 C until biochemical analyses.

Eligibility

Sex: ALLMin age: 35 YearsMax age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria: * newly diagnosed type 2 diabetes and non-diabetic control subjects from 35 to 65 years old were enrolled in this study. Exclusion Criteria: * individuals with inflammatory diseases, Cushing's syndrome, thyroid disorders, infectious diseases, cancers, hepatic diseases and renal impairment. Subjects on insulin or other medications that could affect glucose metabolism or pro-inflammatory cytokines assessment (Glucocorticoids, beta-Adrenergic agonists, Thiazides, etc.....) were also excluded from study

Locations (1)

Tanta University

Tanta, El-Gharbia, Egypt

Outcomes

Primary Outcomes

Fasting blood glucose (FBG) levels

were assayed using glucose oxidase method.

Time frame: three months

Fasting plasma insulin (FPI)

was assayed using Enzyme-Linked Immunosorbent Assay kits i.e ELISA

Time frame: three months

HOMA-IR index

HOMA-IR index which is defined as fasting insulin (μIU/ml) times fasting glucose (mg/L) divided by 405 if fasting blood glucose is expressed in mass units (mg/dl).

Time frame: three months

plasma IL-34

human plasma IL-34 was assayed using sandwich enzyme immunoassay technique

Time frame: Three months

serum visfatin

serum visfatin concentrations enzyme- immunoassay kit

Time frame: Three Months

IRAPe

extracellular part of insulin regulated aminopeptidase (IRAPe) level was determined using sandwich enzyme immunoassay technique

Time frame: Three months

Data from ClinicalTrials.gov

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