Prevalence of HPV-associated Eye Infection and Cytokine Levels in Tears From Patients Diagnosed With Pterygium

Chulalongkorn University141 enrolled

Overview

Prevalence of HPV-associated eye infection and cytokine levels in tears from patients diagnosed with pterygium

The main purpose of this study is to evaluate the prevalence of HPV infection in primary and recurrent pterygium and the association of HPV types collecting from conjunctiva swab and urine. The second purpose is to compare a quantity of cytokines including IL-6, IL-18 and growth factor including VEGF found in tears of patients with primary and recurrent pterygium to participants with free of ocular disease Primary hypothesis \- The prevalence of HPV infection in primary pterygium is difference with prevalence in recurrent pterygium patients. Secondary hypothesis * Ocular HPV infection has association with HPV genitalia infection. * Tears cytokine and growth factor level (IL-6, IL-18 and VEGF) are higher in pterygium HPV infection than non-HPV infection and also more than normal population

Study Type

OBSERVATIONAL

Enrollment

141

Conditions

Human Papilloma Virus Infection Pterygium

Interventions

HPV genotyping assay using HPV GenoArray Diagnostic Kits (Hybribio Ltd., Sheung Wan, Hong Kong)DIAGNOSTIC_TEST

HPV genotyping assay using HPV GenoArray Diagnostic Kits (Hybribio Ltd., Sheung Wan, Hong Kong) for swab sample collected from pterygium and normal conjunctiva and also for urine collected in Colli-PeeTM device (Novosanis, Belgium)

Bio-Plex® 200 system (Bio- Rad, Hercules, CA)DIAGNOSTIC_TEST

Bioplex was use for measure cytokine IL-6, IL-18 and VEGF from tears collected from Schirmer strip

Eligibility

Sex: ALLHealthy volunteers:

Medical Language ↔ Plain English

Participants as primary and secondary pterygium group Inclusion Criteria: * Age above 18 years old * presence of pterygium invading the cornea Exclusion Criteria: * Other ocular inflammation that could affect the results of the study (such as conjunctivitis, keratitis, conjunctival tumor, etc) or would preclude the enrollment for safety reasons Participant without ocular disease Inclusion criteria * Age above 18 years old Exclusion criteria * Presence of any ocular inflammation diseases

Locations (1)

Faculty of medicine, Chulalongkorn university

Bangkok, Thailand

Outcomes

Primary Outcomes

HPV prevalence

1\. To compare HPV prevalence in subjects with primary pterygium and with recurrent pterygium

Time frame: 1 hour per participants which is on a day for collecting sample

Secondary Outcomes

HPV autoinoculation

To assess whether HPV autoinoculation is present, we evaluate whether there is an association between HPV ocular infection and genital infection.

Time frame: 1 hour per participants which is on a day for collecting sample

cytokine IL-6, IL-18 and VEGF levels

To compare cytokine IL-6, IL-18 and VEGF levels among various subgroups, including recurrent pterygium subjects with HPV positive and HPV negative status, primary pterygium subjects with HPV positive and HPV negative, and subjects without ocular diseases

Time frame: 1 hour per participants which is on a day for collecting sample

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.