Time Course of Postprandial Protein Metabolism

University of Illinois at Urbana-Champaign7 enrolled

Overview

The anabolic action of 'fast' whey protein on the regulation of postprandial muscle protein synthesis has been established to be short-lived in healthy young adults. Our aim was assess the time course of anabolic signaling events and stimulation of muscle protein synthesis rates (MPS) after ingestion of a food source that represents a more typical meal-induced pattern of aminoacidemia, namely milk protein concentrate, in healthy young males.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Muscle Protein Synthesis Protein Metabolism

Interventions

Milk Protein ConcentrateDIETARY_SUPPLEMENT

The macronutrient composition and energy of the milk protein beverage provided to participants is 38 g protein (3.46 g leucine), 4.17 g carbohydrate, and 1.4 g fat. The milk protein met all chemical and bacteriologic specifications for human consumption. The L-\[1-13C\]phenylalanine and L-\[1-13C\]leucine enrichments in the milk protein concentrate averaged 38.3 and 10.8 mole percent excess (MPE), respectively.

Eligibility

Sex: MALEMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion criteria * Males * Aged between 18-35 years * Healthy, recreationally active * BMI \< 30 kg/m2 Exclusion criteria * Smoking * Allergies to milk proteins * Vegetarians * Diagnosed GI tract diseases * Female * Arthritic conditions * A history of neuromuscular problems * Previous participation in amino acid tracer studies * Predisposition to hypertrophic scarring or keloid formation * Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).

Locations (1)

Freer Hall

Urbana, Illinois, United States

Outcomes

Primary Outcomes

Fractional synthesis rates of myofibrillar protein

Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion. This will allow us assess the timecourse of muscle protein synthesis after eating.

Time frame: 8 hours

Secondary Outcomes

Phosphorylation of muscle anabolic signaling

Phosphorylation of anabolic signaling pathways (mTORC1, AKT, and P70S6K) involved in the stimulation of myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion.

Time frame: 8 hours

Exogenous rate of phenylalanine appearance

Dietary derived phenylalanine availability will be measured during the 5 h postprandial phase to determine its relationship with the fractional synthesis rates of myofibrillar protein and phosphorylation of muscle anabolic signaling

Time frame: 5 hours

Data from ClinicalTrials.gov

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