This culture system utilizes the special affinity difference of biomedical material coating for different cells to achieve the effect of isolating tumor cells from the blood sample. The coating of the system has the characteristic that to make the WBCs adhesion, but the cancer cells in the blood sample suspend in the culture medium, which achieves the effect of separating cancer cells from the blood. The supernatant with the cancer cells can further be isolated from the cultural system for related analysis and detection to achieve early diagnosis and screening.
Study Type
OBSERVATIONAL
Enrollment
45
National Taiwan University Hospital
Taipei, Taiwan
RECRUITINGThe Number of Circulating Cancer Cells from The Blood Sample
The number of circulating cancer cells from the blood sample will be investigated. To distinguish cancer cells from blood cells for the system, the lung cancer cells were immunofluorescence stained for Cytokeratin, white blood cells were stained for CD45 and nuclei were counterstained with DAPI.
Time frame: about 1 week
Cancer Cell Isolation Efficiency of The Isolation System
The cell isolation efficiency was characterized by spiking A549 lung cancer cells with celltracker at the concentrations 1000, 100 and 10 cells into whole blood.
Time frame: about 1 week
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.