Electronic Hookah and Endothelial Cell Function

University of California, Los Angeles33 enrolled

Overview

Electronic nicotine delivery systems (ENDS) are a rapidly growing global epidemic among adolescents and young adults. Unlike other ENDS such as e-cigarettes, e-hookahs are used through traditional water-pipes, allowing the vapor-containing nicotine, propylene glycol, glycerin, and flavorings-to pass through a water-filled basin, potentially altering the vapor, before it is inhaled through the user's mouth. Contributing to e-hookahs popularity is the belief that the flavored smoke is detoxified as it passes through the water-filled basin, rendering e-hookah a safer tobacco alternative. However, an e-hookahs deliver flavored nicotine by creating a vapor of fine particles and volatile organic compounds that could induce vascular toxicity. While e-hookah vaping acutely reduces endothelial function, the specific role of nicotine and the mechanisms by which it may impairs endothelial function remain understudied. The objective of this project is to investigate the specific role of nicotine in mediating the acute effects of e-hookah vaping on endothelial dysfunction.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

OTHER

Masking

NONE

Enrollment

Conditions

Smoking Endothelial Dysfunction

Electronic hookah vaping with nicotineOTHER

Participants will be invited to vape a 30-minute session of e-hookah containing nicotine

Electronic hookah vaping without nicotineOTHER

Participants will be invited to vape a 30-minute session of e-hookah without containing nicotine

Eligibility

Sex: ALLMin age: 21 YearsMax age: 39 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * 21-39 years old hookah smokers: smoked hookah \>12x in last 12 months * 21-39 years old e-cigarette users: vaped \>12x in last 12 months * no history of illicit drugs * no evidence of cardiopulmonary disease by history/ physical * no diabetes: fasting blood glucose \<100 mg/dl * BP\<140/90mmHg * resting HR\<100 bpm * BMI\<30kg•m2 * no prescription medication Exclusion Criteria: * exhaled CO\>10 ppm (smoking non-abstinence) * positive pregnancy test * psychiatric illness

Locations (1)

University of California, Los Angeles

Los Angeles, California, United States

Outcomes

Primary Outcomes

Flow-Mediated Dilation (FMD)

Using ultrasound, FMD of the brachial artery induced by reactive hyperemia, was used to measure endothelium-dependent vasodilator function. Outcome variable reflecting FMD (brachial artery diameter) was recorded for 45 seconds and resumed 30 seconds before cuff deflation and continuously for 2 minutes after deflation to obtain true peak vasodilatory response.