The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
72
Subjects perform a period of endurance training
Subjects perform a period of resistance training
Subjects perform a period of sprint training
August Krogh Building
Copenhagen, Denmark
Skeletal muscle proteome signature
Pathway enrichment analysis of the muscle proteome
Time frame: Change from baseline at week 6 or 8 depending on the intervention
Anabolic signaling
Western blotting of proteins related to anabolic signaling
Time frame: Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training
Skeletal muscle mitochondrial respiration
High resolution respirometry
Time frame: Change from baseline at week 6 following endurance and sprint training
Maximal oxygen consumption
VO2max during incremental exercise
Time frame: Change from baseline at week 6 following endurance and sprint training
Body composition
Lean and fat mass during dual X-ray absorptiometry
Time frame: Change from baseline at week 6 or 8 depending on the intervention
Myonuclei
Immunohistochemistry to determine number of myonuclei
Time frame: Change from baseline at week 8 following resistance training
Satellite cells
Immunohistochemistry to determine number of satellite cells
Time frame: Change from baseline at week 8 following resistance training
Fiber size
Immunohistochemistry to determine fiber size
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Time frame: Change from baseline at week 8 following resistance training