3-D Super Resolution Ultrasound Microvascular Imaging

Healthy Volunteers Inclusion Criteria * Able to provide informed consent * Negative urine pregnancy test in women of child-bearing potential Exclusion Criteria * Institutionalized subject (prisoner or nursing home patient) * Critically ill or medically unstable and whose critical course during the observation period would be unpredictable (e.g., chronic obstructive pulmonary disease (COPD) * Known hypersensitivity to perflutren lipid (Definity®) * Active cardiac disease including any of the following: * Severe congestive heart failure * Unstable angina. * Severe arrhythmia * Myocardial infarction within 14 days prior to the date of proposed Definity® administration. * Pulmonary hypertension * Cardiac shunts Breast Imaging Patients Inclusion Criteria * Women * Patient had a diagnostic breast ultrasound study performed at UNC * Scheduled for a core needle or surgical breast biopsy of at least one breast lesion that is 2 cm or less in size and 3 cm in depth from the skin surface * Able to provide informed consent * Negative urine pregnancy test in women of child-bearing potential * BIRADS score of 4 or 5. Exclusion Criteria * Male * Institutionalized subject (prisoner or nursing home patient) * Critically ill or medically unstable and whose critical course during the observation period would be unpredictable (e.g., chronic obstructive pulmonary disease (COPD) * Sonographically visible breast lesion larger than 2cm or greater than 3cm in depth from the skin surface * Known hypersensitivity to perflutren lipid (Definity®) * Active cardiac disease including any of the following: * Severe congestive heart failure * Unstable angina. * Severe arrhythmia * Myocardial infarction within 14 days prior to the date of proposed Definity® administration. * Pulmonary hypertension * Cardiac shunts Thyroid Imaging Patients Inclusion Criteria * Patient had a diagnostic thyroid ultrasound study performed at UNC * TIRADS risk score of 4c or 5 * Scheduled for a core needle or surgical thyroid biopsy, fine needle aspiration, or thyroidectomy of at least one sonographically visible thyroid lesion that is 3 cm in depth from the skin surface * Able to provide informed consent * Negative urine pregnancy test in women of child-bearing potential Exclusion Criteria * Institutionalized subject (prisoner or nursing home patient) * Critically ill or medically unstable and whose critical course during the observation period would be unpredictable (e.g., chronic obstructive pulmonary disease (COPD) * Known hypersensitivity to perflutren lipid (Definity®) * Active cardiac disease including any of the following: * Severe congestive heart failure * Unstable angina. * Severe arrhythmia * Myocardial infarction within 14 days prior to the date of proposed Definity® administration. * Pulmonary hypertension * Cardiac shunts