Recent studies have revealed an association between history of suicide attempt and inflammatory markers in both the cerebrospinal fluid and the plasma. Post mortem studies have shown an increase in microglial activation in the brain tissue of suicide victims. However the relationship between peripheral and central inflammation in suicide is probably mediated by complex biological processes that are yet elucidated. An increase of blood S100B levels (biomarker of neurovascular damage; PMID 14530574) has been reported in adolescents with suicidal ideation vs. controls and independently of psychiatric disorder. The investigators hypothesize that peripheral inflammation may alter BBB which normally acts as a filter to ensure proper neuronal functioning in suicidal vs. non suicidal patients.
105 participants will be recruited : * 35 depressed patients having attempted suicide (maximum 3 attempts in life) * 35 depressed patients without history of suicide attempt (affective controls) * 35 healhy controls with no lifetime psychiatric history. Only one visit is planned. A clinical assessment (2 hours) will be performed to characterise pschopathology and suicidal characteristics. Blood and salivary samples will be obtained in order to measure inflammatory markers.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
105
Blood and salivary samples will be taken at inclusion and a psychiatric assessment will be carried out
University hospital
Montpellier, France
RECRUITINGBlood level of S100B
Blood level of S100B measured in 3 groups (i.e. suicide attempters, affective controls, healthy controls)
Time frame: at inclusion
Determination of leukocyte signature associated with suicidal behavior and depression
Comparison of the number and percentage of leukocytes (lymphocytes, monocytes, neutrophils) in the blood between the 3 patients groups
Time frame: at inclusion
Profile of peripheral inflammation of each group
Characterization of activation state of leukocytes of CD4 / CD8 (T cells), NK cells, CD14 / CD33 (monocytes / macrophages)
Time frame: at inclusion
Identification of general peripheral inflammation
Between groups comparison of blood levels of CRP
Time frame: at inclusion
Pro-inflammatory profile based on cytokins of each group
Serum pro-inflammatory interleukins (IL) -1b, IL2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13 , TGF-b, interferon IFN-g, TNF-a, TNF-b
Time frame: at inclusion
Profile of salivary interleukins
Profile of salivary interleukins Il-6, Il-13 et Il-17 within and between groups
Time frame: at inclusion
Serum levels of glial fibrillary actin protein (GFAP)
Between group comparison of serum levels (ng / ml) of glial fibrillary actin protein (GFAP)
Time frame: at inclusion
Serum levels of specific neuronal enolase (NSE)
Between group comparison of serum levels (ng / ml) of specific neuronal enolase (NSE)
Time frame: at inclusion
Serum levels of ubiquitinar-carboxy-terminal hydrolase L1 (UCH-L1)
Between group comparison of serum levels (ng / ml) of ubiquitinar-carboxy-terminal hydrolase L1 (UCH-L1)
Time frame: at inclusion
Salivary levels of S100B protein
Between group comparison of salivary levels of S100B protein
Time frame: at inclusion
Salivary levels of specific neuronal enolase (NSE)
Between group comparison of salivary levels of specific neuronal enolase (NSE) in the 3 groups
Time frame: at inclusion
Salivary levels of GFAP
Between group comparison of salivary levels of GFAP in the 3 groups
Time frame: at inclusion
In vitro fluorescence ratio Dapi / phalloidin
The fluorescence ratio Dapi / phalloidin in a culture of neurovascular cells in the presence of the serum of the subjects (in vitro) in the 3 groups
Time frame: at inclusion
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