Retrospective cohort study for 189 couples assigned to normal and abnormal Sperm DNA fragmentation (SDF) groups.To determine the effect of SDF on blastocyst's trophectoderm (TE), Inner cell mass (ICM) morphology grading, and implantation rate. Semen in both groups was processed by density gradient (DG).
Purpose: to determine if sperm DNA fragmentation (SDF) has a correlation with Blastocyst's trophectoderm (TE) and Inner cell mass (ICM) grading, or with the implantation rate? Method: Retrospective cohort study for 189 couples, assigned to normal SDF group (121 cases), and abnormal SDF group (68 cases). Semen in both groups was processed by double layer density gradient (DG). Statistical analysis done using SPSS version 22.
Study Type
OBSERVATIONAL
Enrollment
189
Intracytoplasmic sperm injection of female partner oocytes with ejaculated sperm selected by density gradient centrifugation
Ganin Fertility Center
Cairo, Egypt
Blastocyst morphological grading
Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A to C ( A is the highest quality).
Time frame: 5-6 days after ICSI
Implantation rate
Percentage of the implanted embryos number for each group / number of transferred embryos.
Time frame: 6 weeks of pregnancy
Fertilization rate
Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes
Time frame: 16-19 hours of ICSI
Blastulation rate
Percentage of total blastocysts / total 2PN
Time frame: 5-6 days of ICSI
Pregnancy rate
Percentage of pregnant cases / transferred cases for each group ( positive pregnancy test)
Time frame: After 2 weeks of embryo transfer
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