The aim of the study is the determination of the zearalenone and its metabolites (α and β) level in the blood plasma and faeces of patients at increased risk of cancer, in relation to the nutrition data (FFQ) and information on the quality of life dependent on health (WHOQOL-BREF). The study is performer within the framework of the Polish Colonoscopy Screening Program. Within the study 100 volunteers between 50 and 65 years of age. while, the colonoscopies will be performed in Provincial Specialist Hospital in Olsztyn. Patients with positive test result, who have been diagnosed with polyps or cancer, will be assigned to risk group. The study has been approved by a local bioethics committee.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
100
colonoscopy
complete the two questionnaires (Food Frequency Questionnaire (FFQ) and the questionnaire of The World Health Organization Quality of Life (WHOQOL-BREF)
completing the demographic questionnaire
For the determination of anthropometric data will be used a professional body mass composition analyser (Seca,Hamburg, Germany)
blood and faeces collections
Provincial Specialist Hospital in Olsztyn
Olsztyn, Poland
RECRUITINGCompletion of the assigned screening strategy
Completion of the assigned screening strategy within 8 weeks of enrollment defined as: \* current screening strategy: documented screening colonoscopy.
Time frame: Within 8 weeks of enrollment
Analysis of the concentration of the mycotoxin
Determination of the concentration and a profile of the mycotoxin using the high-performance liquid chromatography coupled with the mass spectrometry (HPLC-MS)
Time frame: 5 month
Characteristics of gut bacterial
The presence of bacterial DNA in the samples will be confirmed using real-time polymerase chain reaction (RT-PCR). Microbial diversity will be study by sequencing the amplified V3-V4 region of the 16S rRNA gene by using primers 16S. Next, the amplicon pools will be prepared for sequencing and the size and quantity of the amplicon library will be assessed on NGS MiSeq.
Time frame: 6 month
Determination of anthropometric data
The anthropometric data will be determined using the professional body mass composition analyser. Based on the obtained data will be calculated Body Mass Index (BMI), which will be expressed in kg/m\^2
Time frame: 4 weeks
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