Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

Eastern Mediterranean University86 enrolled

Overview

This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect. Therefore, this study hypothesizes that: H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise. H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism. H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels. H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism. H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Uncoupling Protein-1 Polymorphism, Restriction Fragment Length

Eligibility

Sex: MALEMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Athletes group: * Must be basketball, or volleyball, or handball players. * Must be subjects who have been training regularly for at least 3 months. * Sedentary group: \* Must be subjects who have not been training regularly for at least 3 months. * For both groups: * Healthy. * Non obese (body mass index \< 30). * Aged between 18 and 35 years. Exclusion Criteria: * For both groups: * Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances. * Being obese (body mass index \> 30). * Having an acute or chronic injury which is limiting physical performance. * Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples. * Being injured or sick during the study.

Outcomes

Primary Outcomes

Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory.

Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory.

Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Uncoupling protein-1-3826 A/G polymorphism determination

Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory.

Time frame: 15 minutes before the Yo-Yo intermittent recovery test (level 1)

Secondary Outcomes

Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory.

Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)

Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory.

Time frame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes