The investigators will be collecting prostate and fat tissue from participants undergoing radical prostatectomy to culture and study in the laboratory.
Both obesity and prostate cancer are major health problems in the UK, affecting 1 in 3 and 1 in 8 men respectively. Moreover, obesity increases the risk of developing aggressive disease and the risk of prostate cancer spreading. How this happens is not known. The investigators want to understand how fat cells communicate with prostate cancer cells. Investigators will be collecting prostate and fat tissue from radical prostatectomy participants for culture, in particular to look at exosome communication.
Study Type
OBSERVATIONAL
Enrollment
100
Routine NHS Radical Prostatectomy
Charing Cross Hospital, Imperial College Healthcare NHS Trust
London, United Kingdom
RECRUITINGDetermine functional differences of peri-prostatic adipose tissue from lean vs obese patients on prostate cancer cell lines
Proliferation, migration, invasion, apoptosis and epthelial-mesenchymal transition assays will be performed using isolated exosomes on prostate cancer cell lines
Time frame: Through to study completion, on average 3 years
Identification of exosomal small RNAs transferred between human adipose tissue to prostate cancer cells lines
Exosomal RNA transferred from human adipose tissue to prostate cancer cell lines will be isolated using a tagged magnetic bead isolation method and sequenced to identify transferred small RNAs
Time frame: Through to study completion, on average 3 years
Assess how exosomal small RNAs from lean vs obese patients affect cancer regulation
Prostate cancer cell lines will be treated with isolated exosomes, then parental and treated cells sequenced to identify differentially expressed RNA and underlying changes
Time frame: Through to study completion, on average 3 years
Attempt to replicate functional changes observed
Treatment of prostate cancer cell lines with microRNA mimics or inhibitors and comparison of changes with exosome treated cell lines
Time frame: Through to study completion, on average 3 years
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