Skin Tape Transcriptome Methods in Children

Inclusion Criteria: 1. Able to provide informed consent/assent. 2. Male or female, 4-12 years of age (prepuberty) inclusive at the time of consenting 3. Participant must be either: 1. Active atopic dermatitis with two areas of non-lesional AD within 5 cm of the measured lesional area within the same region. OR 2. .No personal history or current manifestations of AD, food allergy, asthma, allergic rhinitis, and no positive prick or blood testing for allergen (based on self-report); and no evidence of dry skin or other skin condition Exclusion Criteria: 1. Unable to provide consent or comply with the protocol 2. Presence of any skin condition that might compromise the stratum corneum barrier in infants and young children (e.g., ichthyosis, psoriasis, extensive seborrheic dermatitis, scabies) 3. History of any skin reaction to tape, or adhesives 4. Past or current medical problems or findings from physical examination or laboratory testing that are not listed above, which in the opinion of the investigator, may pose additional risks from participation in the study, may interfere with the participant's ability to comply with study requirements or that may impact the quality or interpretation of the data obtained from the study. 5. Use of any topical product (e.g., emollient, topical corticosteroids, topical immunomodulatory agents, topical antibiotics) on the area(s) to be tested within 24 hours before the study visit.