Co-infusion of Neurotensin and GLP-1 Effects on Appetite and Food Intake.
Neurotensin (NT), a gut hormone and neuropeptide, and GLP-1, as well a gut hormone, increases in circulation after bariatric surgery in rodents and humans and inhibit food intake in mice. Studies in rodents have shown that a GLP-1 receptor agonist and long acting neurotensin inhibit food intake. This study investigates whether the synergistic anorexic effects of neurotensin and GLP-1 agonism suggested by animal studies can be translated.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
35
Double saline infusion followed by an ad libitum meal
Neurotensin + saline infusion followed by an ad libitum meal
GLP-1 + saline infusion followed by an ad libitum meal
University of Copenhagen, Department of Biomedical Scienses
Copenhagen, Denmark
RECRUITINGCopenhagen University Hospital Hvidovre
Copenhagen, Denmark
RECRUITINGAd-libitum food intake
Neurotensin (NT), saline, GLP-1 and GLP-1+Neurotensin will be infused on four occasions in random order, double blinded. After 2h of infusion an ad libitum meal will be served. When the meal is completed food intake will be determined by weighing the leftovers.
Time frame: 120 min
Appetite and gastrointestinal sensations
Using visual analogue scales (VAS) the effect of infusions and meals will be documented. Ad libitum meals will also be evaluated using VAS.
Time frame: -60, -30, 0, 30, 60, 90, 120, 150 and 180 min
Plasma glucose
Bed-side measurements of plasma glucose
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Neurotensin
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
GLP-1
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Insulin
Serum analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
C-peptide
Serum analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Bile acids
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
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GLP-1 + Neurotensin infusion followed by an ad libitum meal
Ghrelin
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Peptide YY (PYY)
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Leptin
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Metabolomics
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min
Proteomics
Plasma analysis
Time frame: -60, -30, 0, 15, 30, 45, 60, 90, 120, 150, 165 and 180 min