The aim of the study is to investigate dynamic changes in biochemical metabolism, gut microbiome and its metabolites among overweight and obese participants while they are on a modified Atkins Diets.
The mechanisms of obesity are complex, involved in genes, environment, life style, and systemic and adipose tissue inflammation. Dysbiosis of gut microbiome is considered to make a difference in obesity. On the other hand, both long-term and short-term dietary interventions can induce changes in the body composition and structure and function of gut microbiome. However, it is not really clear how gut microbiome changes along with weight loss in overweight or obese. In the present study, investigators aim to characterize the gut microbiome among overweight or obese participants on a modified Atkins diets. The stool and blood samples were collected by an interval of every 5% and 10% of weight loss, respectively. Data collecting mainly covers: 1) body composition analysis ; 2) obesity related biochemical metabolism; 3) measurement of gut microbiome using metagenome and 16S rRNA sequencing techniques; 4) metabolomics of serum and gut microbiome; 5) measurement of physical activity and diets using questionnaires.
Study Type
OBSERVATIONAL
Enrollment
75
Hebei Medical University Third Hospital
Shijiazhuang, Hebei, China
Dynamic changes of gut microbiome
The stool sample will be collected by an interval of each 5% weight loss that participants experienced. Fecal microbiota will be analyzed by 16S rRNA gene sequencing technique.
Time frame: 36 months
Dynamic changes in faecal metabolomics
For participants achieving each 10% weight loss, targeting metabonomics will be applied to identify the abundances of faecal metabolites (e.g. short-chain fatty acids, amino acids and carbohyrates) using validated mass spectrometry methods.
Time frame: 36 months
Dynamic changes of body mass index
Weight and height will be combined to report BMI in kg/m\^2.
Time frame: 36 months
Dynamic changes of body fat
As a body composition parameter, body fat will be analyzed by body composition automatic analyzer for each participant every 10-20 days.
Time frame: 36 months
Dynamic changes of muscle mass
Muscle mass will be analyzed by body composition automatic analyzer for each participant every 10-20 days.
Time frame: 36 months
Dynamic changes of blood lipids
For participants achieving each 10% weight loss, intravenous serum will be collected and the parameters which can reflect the lipids metalism (such as triglyceride, total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol ) will be measured by automatic biochemical analyzer.
Time frame: 36 months
Dynamic changes of fasting blood glucose
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For participants achieving each 10% weight loss, intravenous serum will be collected and fasting blood glucose will be measured by automatic biochemical analyzer.
Time frame: 36 months
Dynamic changes of homocysteine
For participants achieving each 10% weight loss, intravenous serum will be collected and homocysteine will be measured by automatic biochemical analyzer.
Time frame: 36 months
Dynamic changes of blood pressure
For participants achieving each 10% weight loss, blood pressure will be measured by the standard electronic sphygmomanometer.
Time frame: 36 months