The aim of this study is to investigate whether smoking is associated with changes in salivary composition and/or predisposition to erosion. Healthy volunteers are observationally wearing an intraoral device with both bovine tooth specimens (enamel and dentin) and resin specimens twice for two hours each. Afterwards, specimens are eroded extraorally and calcium release into the acid is measured. Total protein concentration and protein composition of the salivary pellicles on the resin samples are measured. Additionally, salivary parameters (unstimulated and stimulated saliva flow rate, pH, buffer capacity, total protein content and protein composition as well as concentration of inorganic calcium, phosphate, and fluoride) are measured.
Study Type
OBSERVATIONAL
Enrollment
50
Use of fluoridated toothpaste
Wearing of an intraoral device with bovine tooth samples
Wearing of an intraoral device with resin samples
University Medical Center Göttingen, Dept. of Preventive Dentistry, Periodontology and Cariology
Göttingen, Lower Saxony, Germany
Calcium release from bovine enamel and dentin specimens by extraoral erosion (nmol/Square Millimeter).
Time frame: Immediately after the intraoral device has been worn once for two hours.
Determination of unstimulated and stimulated saliva flow rate (mL/min).
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of saliva pH (pH).
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of salivary buffer capacity (pH).
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of total protein content (mg/L) and protein composition (qualitatively) in saliva.
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of inorganic calcium (mmol/L), phosphate (mmol/L) and fluoride (µmol/L) in saliva.
Time frame: Saliva samples are collected for 5 min each on three visits.
Determination of total protein concentration (ng/Square Millimeter) and protein composition (qualitatively) within the salivary pellicles.
Time frame: Immediately after the intraoral device with resin samples has been worn once for two hours.
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