The present project aims to investigate the effects of high-quality protein (whey protein) vs. low-quality protein (collagen) on muscle mass and function (e.g., strength and power) during a 10-wk resistance training program in young adults. It will be tested the hypothesis that leucine-matched collagen should promote similar gains on muscle function and mass compared to whey protein supplementation.
The purpose of this study will be to investigate the effects of whey protein vs. leucine-matched collagen supplementation on muscle mass and function after a 10-wk resistance training program. Initially, all participants will be submitted for a 2-wk familiarization period, and then randomized into two groups (N = 11 / group): whey protein group and leucine-matched collagen group. The following variables will be assessed from pre-to post-training: anthropometric, muscle thickness from vastus lateralis and biceps brachii muscles using ultrasound, training load, mean power and peak torque for unilateral arm flexion exercise using a isokinetic dynamometer, countermovement vertical jump, nutritional intake, and testosterone-to-cortisol ratio . Shapiro-Wilk and Levine tests will be used to test the normality and homogeneity of the measurements, respectively. Two-way ANOVA (group x time) tests for repeated measures will be performed to assess changes over time and between groups for all dependent variables. Violation of sphericity will adjust using Greenhouse- Geyser correction. The Bonferroni post-hoc test will detect specific differences between groups. When appropriate, the percentage of change (∆%) will be reported according to the following equation: ∆% = \[(pre-average post average) / pre-average mean\] × 100. Values will be express as mean ± standard deviation. O significance level α will be 0.05.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
22
35 g of whey protein (7 d/wk) during a 10-wk resistance training program (3 d/wk; 3 sets of 8-12 repetitions with an interval of 1-2 minutes between sets and exercises) involving the leg press, leg extension, and arm flexion exercises. The load will be adjusted weekly.
35 g of collagen (7 d/wk) + 2 g of leucine during a 10-wk resistance training program (3 d/wk; 3 sets of 8-12 repetitions with an interval of 1-2 minutes between sets and exercises) involving the leg press, leg extension, and arm flexion exercises. The load will be adjusted weekly.
Universidade Norte do Paraná
Londrina, Paraná, Brazil
Change in biceps brachii thickness
Measured by ultrasonography pre- and post-training
Time frame: Baseline and after 10 weeks
Change in muscle strength by training load
Training load for leg press, knee extension, and arm flexion exercises
Time frame: Baseline, 5 and 10 weeks
Change in vertical jump
Countermovement vertical jump performance will be assessed using the Smart Jump instrument
Time frame: Baseline and after 10 weeks
Change in mean power
Mean power using a isokinetic dynamometer (BIODEX)
Time frame: Baseline and after 10 weeks
Change in peak torque
Peak torque using a isokinetic dynamometer (BIODEX)
Time frame: Baseline and after 10 weeks
Change in vastus lateralis thickness
Measured by ultrasonography pre- and post-training
Time frame: Baseline and after 10 weeks
Change in testosterone-to-cortisol ratio
Blood samples will be collected for analyse of the testosterone-to-cortisol ratio
Time frame: Baseline and after 10 weeks
Dietary intake
Dietary intake to be assessed using 3-day diet recalls.
Time frame: Baseline, 2, 5, 10 weeks
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