Investigating the Effect of Obesity on Eosinophil Recruitment in Human Adipose Tissue

Mayo Clinic24 enrolled

Overview

Researchers are trying to better understand the relationship between inflammation in adipose tissue (AT) and insulin resistance.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Obese Healthy

Interventions

Fat Tissue BiopsyPROCEDURE

If subjects are scheduled for a surgical procedure a fat sample will be collected during the beginning of the surgery. If subjects are not scheduled for a surgical procedure, a small sample of subcutaneous (superficial) fat tissue from subjects abdomen area will be collected.

Eligibility

Sex: ALLMin age: 30 YearsMax age: 50 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Adults ages 30-50 years * Non-smokers * Lean subjects with a BMI of 25 or less, gender specific normal body fat, * Obese, non-diabetic subjects with a BMI between 30 and 50 and taking no medications affecting glucose metabolism or lipid metabolism * subjects scheduled for an elective laparoscopic or laparotomy abdominal surgery Exclusion Criteria: * Receiving corticosteroid therapy or have a history of asthma, COPD or atopic syndrome * taking any biologic treatment for any autoimmune disorder * Taking any leukotriene receptor antagonist acting medications (ex. Montelukast) * smoking

Locations (1)

Mayo Clinic

Scottsdale, Arizona, United States

Outcomes

Primary Outcomes

Number of eosinophils in adipose tissue

eosinophil number will be measured by flow cytometry in subcutaneous (superficial) adipose tissue

Time frame: baseline

Secondary Outcomes

Correlation between eosinophil content and body mass index (BMI)

Measured as a linear regression between BMI (defined as the weight in Kg/height in square m; kg/m2) and eosinophil number in the fat

Time frame: baseline

Data from ClinicalTrials.gov

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