The dietary shift from animal to plant protein sources is one of the key aspects of the nutritional transition towards more sustainable food system and diets. However the metabolic implication of this shift in protein sources are still poorly understood. This project aims to characterize and understand the metabolic orientations specifically induced by animal and vegetable dietary proteins, in order to better analyze the metabolic reorientations that would result from the expected increase in the share of plant proteins in different dietary contexts, especially those of the Western type, often associated with the development of metabolic deregulations (obesity and cardiometabolic risk).
The main objectives of this project are: * Characterize the metabolic adaptations induced by animal or plant protein diets and their repercussions in terms of physiology and health. * Characterize the medium-term metabolomic signatures induced by this shift in dietary protein sources * Validate, in a human population, biomarkers of dietary animal or plant proteins, previously identified in pre-clinical studies. This clinical trial is open, monocentric, controlled, randomized, with a cross experimental design. 20 men or postmenopausal women will follow for 4 weeks a controlled diet with a protein fraction constituted mainly from animal or vegetal sources. After a 2-week washout period(+21D/-7D), they will follow another 4 week of controlled diet with predominantly animal or plant protein depending on 1st intervention period diet. At the end of each intervention period, a post-prandial exploration will be conducted with the administration of a high-fat, high-sugar meal and subsequent blood and urine sampling. The order in which participants will received the two diets will be randomized.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
53
20 men or postmenopausal women will follow for 4 weeks a controlled diet with a protein fraction constituted mainly from animal sources. At the end of the intervention period, a post-prandial exploration will be conducted with the administration of a high-fat, high-sugar meal and subsequent blood and urine sampling.
20 men or postmenopausal women will follow for 4 weeks a controlled diet with a protein fraction constituted mainly from vegetal sources. At the end of the intervention period, a post-prandial exploration will be conducted with the administration of a high-fat, high-sugar meal and subsequent blood and urine sampling.
CHU de Clermont-Ferrand
Clermont-Ferrand, France
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 0
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 14
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 28
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 29
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 42
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 56
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 70
changes of blood metabolomics
the plasma metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 71
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 0
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 14
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 28
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 29
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 42
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 56
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 70
Changes of urine metabolomics
the urine metabolome will be determined by Liquid Chromatography - Mass Spectrometry
Time frame: day 71
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 0
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 14
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 28
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 29
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 42
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 56
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 70
Changes in blood glucose
The glucose concentrations will be determined by the blood samples taken by ELISA
Time frame: day 71
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 0
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 14
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 28
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 29
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 42
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 56
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 70
Changes in blood insulin
The insulin concentrations will be determined in the blood samples and measured by ELISA
Time frame: day 71
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 0
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 14
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 28
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 29
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 42
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 56
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 70
Changes in blood cholesterol
The cholesterol concentrations will be determined in the blood samples taken
Time frame: day 71
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 0
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 14
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 28
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 29
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 42
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 56
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 70
Changes in blood triglycerides
The triglycerides concentrations will be determined in the blood samples taken
Time frame: day 71
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 0
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 14
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 28
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 29
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 42
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 56
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 70
changes in blood IL-6
The IL-6 concentrations will be determined in the blood samples taken
Time frame: day 71
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 0
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 14
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 28
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 29
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 42
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 56
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 70
changes in blood IL-10
The IL-10 concentrations will be determined in the blood samples taken
Time frame: day 71
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 0
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 14
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 28
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 29
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 42
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 56
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 70
changes in blood CRP
The CRP concentrations will be determined in the blood samples taken
Time frame: Day 71
measure of protein synthesis by isotopic labelling
measurement of protein synthesis using deuterium labelling water
Time frame: Day 28
measure of protein synthesis by isotopic labelling
measurement of protein synthesis using deuterium labelling water
Time frame: Day 29
measure of protein synthesis by isotopic labelling
measurement of protein synthesis using deuterium labelling water
Time frame: Day 70
measure of protein synthesis by isotopic labelling
measurement of protein synthesis using deuterium labelling water
Time frame: Day 71
Measure of lipogenesis de novo by isotopic labelling
measurement of lipogenesis using deuterium labelling water
Time frame: Day 28
Measure of lipogenesis de novo by isotopic labelling
measurement of lipogenesis using deuterium labelling water
Time frame: Day 29
Measure of lipogenesis de novo by isotopic labelling
measurement of lipogenesis using deuterium labelling water
Time frame: Day 70
Measure of lipogenesis de novo by isotopic labelling
measurement of lipogenesis using deuterium labelling water
Time frame: Day 71
Changes in vascular function
will be determined by measuring minimal and maximal diameter of brachial artery in mm and the percentage of dilatation using the Flow-Mediated Dilatation GE echographer
Time frame: Day 0
Changes in vascular function
will be determined by measuring minimal and maximal diameter of brachial artery in mm and the percentage of dilatation using the Flow-Mediated Dilatation GE echographer
Time frame: Day 28
Changes in vascular function
will be determined by measuring minimal and maximal diameter of brachial artery in mm and the percentage of dilatation using the Flow-Mediated Dilatation GE echographer
Time frame: Day 42
Changes in vascular function
will be determined by measuring minimal and maximal diameter of brachial artery in mm and the percentage of dilatation using the Flow-Mediated Dilatation GE echographer
Time frame: Day 70
Changes in microcirculation
will be determined measuring resting state and maximal flow by Flow Laser Doppler Periflux 5000
Time frame: Day 0
Changes in microcirculation
will be determined measuring resting state and maximal flow by Flow Laser Doppler Periflux 5000
Time frame: Day 28
Changes in microcirculation
will be determined measuring resting state and maximal flow by Flow Laser Doppler Periflux 5000
Time frame: Day 42
Changes in microcirculation
will be determined measuring resting state and maximal flow by Flow Laser Doppler Periflux 5000
Time frame: Day 70
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 0
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 14
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 28
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 29
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 42
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 56
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 70
Changes in mRNA (transcriptomics) derived from Peripheral Blood Monocellular Cells (PBMC)
will be measured by qPCR
Time frame: Day 71
Changes in body composition
will be determined using bioelectric impendence analysis, Quad Scan.
Time frame: Day 0
Changes in body composition
will be determined using bioelectric impendence analysis, Quad Scan.
Time frame: Day 28
Changes in body composition
will be determined using bioelectric impendence analysis, Quad Scan.
Time frame: Day 42
Changes in body composition
will be determined using bioelectric impendence analysis, Quad Scan.
Time frame: Day 70
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 0
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 14
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 28
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 29
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 42
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 56
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 70
Changes of the microbiota (stool samples)
will be determined by the identification of bacterial biodiversity by a genetic sequencing analysis of bacterial DNA
Time frame: day 71
Food statement at inclusion
using 3 days food log before day 0
Time frame: day 0
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