A randomized, double-blind, crossover dietary intervention trial will test the effects of 4 weeks of daily honey-flavored yogurt intake on markers of inflammation (Th17 cytokines) and oxidative stress (NOX2, UA, RSNO) and associative changes with microbial derived metabolites (SCFAs, BAs, ellagitannins), metabolism and the fecal microbiome. The above suite of selected markers will capture diet-induced systemic changes in inflammation and oxidative stress, while assessing associated microbial changes.
Those qualified for enrollment will be randomized into a double-blind crossover study to consume 2 morning servings of a 0.6 cup (150g) of plain yogurt with : A) cane sugar added in an isocaloric level as the honey or, B) 1 tbsp of phenolic-rich honey for 4 weeks. A one-month washout between periods will be used. The participants will be instructed to consume one yogurt in replacement of or as part of breakfast, and as a late morning snack. They will also be instructed to not add any additional items to the yogurt. Therefore, each study participant will consume 2 tbsp. of honey a day for four weeks, which is a realistic amount typically consumed by honey users.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
SINGLE
Enrollment
20
2 tbsp. of daily honey intake in 1.2 cups of yogurt per day, split into two equal portions
sugar added, in an isocaloric fashion to the honey, to 1.2 cups of yogurt per day, split into two equal portions
University of California, Davis; Department of Nutrition
Davis, California, United States
RECRUITINGConcentration of CD4+ T Helper (TH)17 cytokines
Cytokines included in the panel are: Il-1b, IL-4, IL-6, IL-10, IL-17a, IL-21, IL-22, IL-23, IL-31, IL-33, IFN-gamma, TNF-alpha, IL-17f.
Time frame: 4 weeks
Concentration of Secondary bile acids
plasma microbial metabolites
Time frame: 4 weeks
Concentration of Short chain fatty acids
Fecal microbial metabolites
Time frame: 4 weeks
Concentration of urolithins and other ellagitannin-derived metabolites
microbial metabolites
Time frame: 4 weeks
Concentration of soluble NADPH oxidase (NOX2)
plasma marker oxidative stress
Time frame: 4 weeks
Concentration of uric acid
plasma marker oxidative stress
Time frame: 4 weeks
Concentration of total Nitrate/Nitrite and Nitric Oxide related metabolites (RSNO)
dietary nitrate and NO metabolites
Time frame: 4 weeks
Concentration of untargeted metabolomics
metabolism
Time frame: 4 weeks
Determination of microbial populations
stool bacterial population
Time frame: 4 weeks
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