The objective of the study is to determine RNA blood biomarker based on 9 genes already identified in experimental studies, whose expression would be significantly increased in patient with ischemic stroke compared to controls.
This is a monocentric study. It's a case-control study with case being first-ever ischemic stroke and controls being healthy (stroke free) subject patients paired for age, sex and cardiovascular risk or hemorrhagic stroke paired for age and sex. Patients are followed for one year. Blood samples will be taken at inclusion, 12h, 24h, 48h, 7d, 3m and 1y. Microvesicles samples will be taken at inclusion, 7d and 3M.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
OTHER
Masking
NONE
Enrollment
61
Blood samples will be taken at inclusion, 12h, 24h, 48h, 7d, 3m and 1y for patients ; at inclusion, 3m and 1y for controls. Microvesicles samples will be taken at inclusion, 7d and 3M ; at inclusion and 3M for controls.
MRI at inclusion for controls
CHRU Brest
Brest, France
Expression (measured in log2) of each 9 targeted genes identified less than 6 hours after inclusion
Each targeted genes will be measured by quantitative reverse transcription Polymerase Chain Reaction (rt-PCR)
Time frame: 6 hours after inclusion
RNA level expression across time according growth of infarction measured at inclusion and at 3 months
Each targeted genes will be measured by quantitative rt-PCR
Time frame: inclusion and 3 months
RNA level expression across time according Rankin scale at three months dichotomized in good (≤ 2) and bad prognosis (> 3).
Each targeted genes will be measured by quantitative rt-PCR
Time frame: at 3 months
Targeted RNA level expression according mechanism of ischemic stroke
Each targeted RNA level expression of targeted genes will be measured by quantitative rt-PCR
Time frame: at 3 months
Target RNA level expression in the saliva
For 3 participants, one of each arm, a saliva sample will be collected at inclusion.
Time frame: at inclusion
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.