Mushroom Ingestion Study

Purdue University7 enrolled

Overview

The investigators propose to determine the effect of consuming 0, 1 or 2 servings of white button (Agaricus bisporus; most popular, lower ergothioneine) or yellow oyster (Pleurotus citrinopileatus; higher ergothioneine) mushrooms on postprandial changes in plasma levels and urinary excretions of mushroom-specific compounds.

The investigators hypothesize that consuming different mushroom varieties will elicit distinct postprandial nutrimetabolomic profiles in the plasma and urine and that the amount of metabolites present will increase with the servings of mushrooms. Measuring ergothioneine in the plasma will provide a quantitative outcome to document the postprandial responses to consuming the test meals. The nutrimetabolomic related outcomes are exploratory and have not been documented in human research.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

OTHER

Masking

SINGLE

Enrollment

Conditions

Diet, Healthy

Interventions

Yellow Oyster -- 84 gOTHER

Participants will consume the standardized test day salad plus 84 g (raw weight) mushrooms. Mushrooms will be cooked prior to being served

Yellow Oyster -- 168 gOTHER

Participants will consume the standardized test day salad plus 168 g (raw weight) mushrooms. Mushrooms will be cooked prior to being served

White button -- 84 gOTHER

Participants will consume the standardized test day salad plus 84 g (raw weight) mushrooms. Mushrooms will be cooked prior to being served

Eligibility

Sex: ALLMin age: 30 YearsMax age: 69 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Male or female; * age 30-69 y; * BMI: 25.0-34.9 kg/m2; * Not severely or extremely depressed (Beck's Depression Inventory score ≤30) * Total cholesterol \<240 mg/dL, low-density lipoprotein cholesterol \<160 mg/dL, triglycerides \<400 mg/dL, fasting glucose \<110 mg/dL; * Systolic/diastolic blood pressure \<140/90 mm Hg; * Body weight stable for 3 months prior (± 3 kg); * Stable physical activity regimen 3 months prior; * Medication use stable for 6 months prior; * Non-smoking; * Non-diabetic; * Not acutely ill; * Females not pregnant or lactating; * Participants must be willing and able to consume the prescribed diets and travel to testing facilities. Exclusion Criteria: * BMI \<25 or \>35; * Severely depressed (Beck's Depression Inventory score \>30); * Total cholesterol \>240 mg/dL, low-density lipoprotein cholesterol \>160 mg/dL, triglycerides \>400 mg/dL, fasting glucose \>110 mg/dL; * Body weight changes in previous 3 months (±3 kg); * Changes in physical activity regimen in the previous 3 months; * Medication changes in the previous 6 months; * Smoking; * Diabetic; * Acute illness; * Pregnant or lactating; * Allergic to mushrooms

Locations (1)

Purdue University

West Lafayette, Indiana, United States

Outcomes

Primary Outcomes

Change in plasma glucose

Plasma will be assessed for glucose values prior to salad consumption and in response to the test day meal

Time frame: 5 hours

Change in plasma insulin

Plasma will be assessed for insulin values prior to salad consumption and in response to the test day meal

Time frame: 5 hours

Plasma metabolomics

Plasma will be assessed for metabolomics (i.e. ergothioneine) via reverse phase liquid chromatography/mass spectrometry

Time frame: 5 hours

Plasma ergothioneine

Plasma will be assessed for ergothioneine concentration, a compound unique to mushrooms

Time frame: 5 hours

Plasma triglycerides

Plasma will be assessed for triglyceride values at the time of salad consumption and at each hour for 5 hours after salad consumption

Time frame: 5 hours

Data from ClinicalTrials.gov

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