Analysis of T Cell Metabolism in Acute Myeloid Leukemia Patients

University of Freiburg77 enrolled

Overview

The objective of this study was to analyze the T cell metabolism and immune phenotype in AML patients during the course of the disease before and after allo-HCT.

Patients who experience relapse of acute myeloid leukemia (AML) after allogeneic hematopoietic cell transplantation (allo-HCT) have a dismal prognosis. Infusion of donor T cells can induce graft-versus-leukemia (GVL) effects, indicating the importance of intact T cell function for leukemia control. Recent studies have shown the importance of metabolic fitness for an effective T cell response. In this study we have analyzed the T cell metabolism and immune phenotype in AML patients at different time points before and after allo-HCT.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Acute Myeloid Leukemia

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * confirmed diagnosis of AML * age ≥ 18 years * peripheral blood sample available * written informed consent * ability to understand the nature of the study and the study related procedures and to comply with them Exclusion Criteria: * age \< 18 years * lack of informed consent * patients that cannot be classified in one of the 3 groups

Outcomes

Primary Outcomes

T cell metabolism

Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) determined by live-cell metabolic assay using the Seahorse Analyzer

Time frame: 4 years

T cell phenotype

T cell cytokine and effector molecule production

Time frame: 4 years

Secondary Outcomes

Metabolite abundance in serum

Serum metabolites as measured by mass spectrometry

Time frame: 4 years

Data from ClinicalTrials.gov

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