Characterization of Methylation Patterns in Hepatocellular Carcinoma

Inclusion Criteria - Case Group: * Age ≥ 22 years * Subjects who are willing and able to provide written informed consent * Subjects with confirmed diagnosis of HCC, naïve to tumor directed therapy Exclusion Criteria - Case Group: * Subjects with current cancer of any kind, other than hepatocellular carcinoma * Subjects with a history of cancer of any kind (including hepatocellular carcinoma), other than non-melanoma skin cancer completely resected * Coinfection with HIV * Prior solid organ transplant Inclusion Criteria - Control Group: * Age ≥ 22 years * Subjects who are willing and able to provide written informed consent * Subjects diagnosed with one of the following: i. Subjects with HCV and cirrhosis ii. Subjects with HBV and cirrhosis iii. Non-cirrhotic chronic HBV subjects at intermediate or high risk of HCC, according to EASL Clinical Practice Guidelines for the management of hepatocellular carcinoma iv. Subjects with Non-Alcoholic Fatty Liver Disease (NAFLD) with cirrhosis. v. Cirrhotic patients due to any other reasons, including alcohol disease * Subjects currently undergoing surveillance for hepatocellular carcinoma * HCC surveillance imaging (e.g., US, CT, MRI) performed within 3 months prior to baseline visit * Subjects with compensated liver function as measured by Child-Pugh Score A or B7 without ascites (all measures should be from the last three months) Exclusion Criteria - Control Group: * Subjects with current cancer of any kind, other than hepatocellular carcinoma * Subjects with a history of cancer of any kind (including hepatocellular carcinoma), other than non-melanoma skin cancer completely resected * Coinfection with HIV * Prior solid organ transplant * Current substance abuse