Characterization of Methylation Patterns in Cancer and Non-Cancer cfDNA

Inclusion Criteria - Cases: 1. Age ≥ 22 years 2. Subjects who are willing and able to provide written informed consent. 3. Subjects with confirmed diagnosis of cancer (primary or recurrent) at all stages, however did not yet undergo any surgery, chemotherapy, radiation or any other treatment for this cancerous lesion (including, but not limited to systemic therapies). Recent cancer staging should be within 60 days prior to baseline visit. Exclusion Criteria - Cases: 1. Known prior diagnosis of cancer except of: 1. Fully resected non-melanoma skin cancer 2. History of the original cancer in cases of recurrent disease. 2. Current co-diagnosis of another type of cancer. 3. Currently receiving, or ever received, any of the following therapies to treat their current cancer: surgical management of the cancer beyond that required to establish the cancer diagnosis; local, regional or systemic chemotherapy including chemoembolization; targeted therapy, immunotherapy including cancer vaccines; hormone therapy; or radiation therapy 4. Pregnancy (by self-report) 5. Current febrile illness 6. Acute exacerbation or flare of an inflammatory condition requiring escalation in medical therapy within 14 days prior to blood draw. 7. Recipient of organ transplant or prior non-autologous (allogeneic) bone marrow or stem cell transplant Inclusion Criteria - Controls: 1. Age 45 to 80 years 2. Subjects who are willing and able to provide written informed consent Exclusion Criteria - Controls: 1. Known current or prior diagnosis of cancer except of fully resected non-melanoma skin cancer 2. Currently in work-up due to suspicion of cancer of any kind 3. Oral or IV corticosteroid use in past 14 days prior to blood draw 4. Pregnancy (by self-report) 5. Current febrile illness 6. Acute exacerbation or flare of an inflammatory condition requiring escalation in medical therapy within 14 days prior to blood draw. 7. Recipient of organ transplant or prior non-autologous (allogeneic) bone marrow or stem cell transplant 8. Life expectancy \< 24 months