ACTH Stability on Whole Blood

University Hospital, Rouen10 enrolled

Overview

ACTH is a peptide secreted by pituitary gland and plays an important role in regulating cortisol secretion. ACTH is determined in plasma by immunoassays using specific antibodies. Its determination is difficult because of instability in whole blood. Several factors which influence ACTH stability in blood before analysis have been identified: temperature, hemolysis, time to centrifugation and presence of protease inhibitors. Published results on ACTH whole blood stability seem contradictory. The objective of this study is to evaluate the effect of aprotinin in 10 healthy volunteers. ACTH measurements will be performed on cobas e602 (Roche Diagnostics, Mannheim, Germany).

Study Type

INTERVENTIONAL

Allocation

Purpose

OTHER

Masking

NONE

Enrollment

Conditions

ACTH

Interventions

Blood samplingBIOLOGICAL

blood sampling is done on healthy volunteers

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Adult patient aged 18 to 60, Exclusion Criteria: * Adult with insufficient venous capital for blood collection

Locations (1)

Rouen University Hospital

Rouen, France

Outcomes

Primary Outcomes

ACTH concentration in plasma

Time frame: 8 hours

Data from ClinicalTrials.gov

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